Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins.
This review summarizes recent studies that define structural domains for DNA binding and transcriptional activation functions in sequence-specific transcription factors in mammalian DNA binding transcription factors.
The genome of the choanoflagellate Monosiga brevicollis and the origin of metazoans
It is shown that the physical linkages among protein domains often differ between M. brevicollis and metazoans, suggesting that abundant domain shuffling followed the separation of the choanoflagellate and metazoan lineages.
Control of cell number by Drosophila FOXO: downstream and feedback regulation of the insulin receptor pathway.
The identification and functional characterization of the Drosophila forkhead-related transcription factor d FOXO is reported, establishing dFOXO as a key transcriptional regulator of the insulin pathway that modulates growth and proliferation.
The activities of two Ets-related transcription factors required for drosophila eye development are modulated by the Ras/MAPK pathway
Transcription regulation and animal diversity
Comparative genome analyses reveal a surprising constancy in genetic content: vertebrate genomes have only about twice the number of genes that invertebrates have, and the increase is primarily due to the duplication of existing genes rather than the invention of new ones.
Affinity purification of sequence-specific DNA binding proteins.
This method provides a means for the purification of rare sequence-specific DNA binding proteins, such as Sp1 and CAAT-binding transcription factor, by using tandem affinity columns containing different protein binding sites.
Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection
It is shown that bacterial C2c2 possesses a unique RNase activity responsible for CRISPR RNA maturation that is distinct from its RNA-activated single-stranded RNA degradation activity.
Orchestrated response: a symphony of transcription factors for gene control.
The question of how gene expression is regulated in complex eukaryotic genomes has re-focused on the molecular machines that have evolved to navigate through chromatin and mediate transcriptional control.
The promoter-specific transcription factor Sp1 binds to upstream sequences in the SV40 early promoter