• Publications
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Bacterial phospholipases C.
  • R. Titball
  • Biology, Medicine
  • Microbiological reviews
  • 1 June 1993
A greater understanding of the modes of action and structure-function relationships of phospholipases C will facilitate the interpretation of studies in which these enzymes are used as membrane probes and will enhance the use of these proteins as models for eukaryotic phospholIPases C. Expand
Structure of the key toxin in gas gangrene
A possible mechanism for α-toxin attack on membrane-packed phospholipid is described, which rationalizes its toxicity when compared to other, non-haemolytic, but homologousospholipases C. Expand
Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens.
Significant differences between the strains include numerous novel mobile elements and genes encoding metabolic capabilities, strain-specific extracellular polysaccharide capsule, sporulation factors, toxins, and other secreted enzymes, providing substantial insight into this medically important bacterial pathogen. Expand
Occurrence of Clostridium perfringens β2-toxin amongst animals, determined using genotyping and subtyping PCR assays
A significant association between C. perfringens possessing the β2-toxin gene and diarrhoea in piglets was identified, suggesting that β2 -toxin may play a key role in the pathogenesis of the disease. Expand
Lipoproteins of Bacterial Pathogens
A general overview of lipoprotein biogenesis is given and examples of the roles oflipoproteins in bacterial disease caused by a selection of medically relevant Gram-negative and Gram-positive pathogens are highlighted. Expand
Molecular Architecture and Functional Analysis of NetB, a Pore-forming Toxin from Clostridium perfringens*
The crystal structure of the pore form of NetB is presented, showing that NetB does not bind phosphocholine efficiently but instead interacts directly with cholesterol leading to enhanced oligomerization and pore formation and providing potential new tools to the field of bionanotechnology. Expand
Genotyping and Phenotyping of Beta2-Toxigenic Clostridium perfringens Fecal Isolates Associated with Gastrointestinal Diseases in Piglets
A significant association between CPB2-positive C. perfringens isolates and diarrhea in piglets is found and is supported by genotyping and phenotyping. Expand
The Clostridium perfringensα-toxin
The gene encoding the α-(cpa) is present in all strains of Clostridium perfringens , and the purified α-toxin has been shown to be a zinc-containing phospholipase C enzyme, which is preferentiallyExpand
The Clostridium perfringens alpha-toxin.
The gene encoding the alpha-(cpa) is present in all strains of Clostridium perfringens, and the purified alpha-toxin has been shown to be a zinc-containing phospholipase C enzyme, which isExpand
Molecular basis of toxicity of Clostridium perfringens epsilon toxin
The exquisite specificity of the toxin for specific cell types suggests that it binds to a receptor found only on these cells, and the crystal structure of ε‐toxin reveals similarity to aerolysin from Aeromonas’hydrophila, parasporin‐2 from Bacillus’thuringiensis and a lectin from Laetiporus’sulphureus. Expand