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Quantitative PCR with 16S rRNA-Gene-Targeted Species-Specific Primers for Analysis of Human Intestinal Bifidobacteria
A highly sensitive quantitative PCR detection method has been developed and applied to the distribution analysis of human intestinal bifidobacterium by combining real-time PCR with BifidOBacterium genus- and species-specific primers, and it was found that the method was applicable to the detection of B ifidobacteria in feces.
Distribution of Bifidobacterial Species in Human Intestinal Microflora Examined with 16S rRNA-Gene-Targeted Species-Specific Primers
Qualitative detection of the bifidobacterial species present in human feces can be accomplished rapidly and accurately and the species-specific PCR method was found to be a useful means for identifying B ifidobacteriumstrains isolated from human feces.
Use of 16S rRNA Gene-Targeted Group-Specific Primers for Real-Time PCR Analysis of Predominant Bacteria in Human Feces
16S rRNA gene-targeted group-specific primers were designed and validated for specific detection and quantification of the Clostridium leptum subgroup and the Atopobium cluster and revealed that the composition of the flora of each volunteer remained stable throughout the test period.
Development of 16S rRNA-Gene-Targeted Group-Specific Primers for the Detection and Identification of Predominant Bacteria in Human Feces
The goal for this specific PCR technique is to develop a procedure for quantitative detection of these bacterial groups, and a real-time quantitative PCR for detection of Bifidobacterium is now being investigated.
Early administration of Bifidobacterium breve to preterm infants: randomised controlled trial
B breve can colonise the immature bowel very effectively and is associated with fewer abnormal abdominal signs and better weight gain in VLBW infants, probably as a result of stabilisation of their intestinal flora and accelerated feeding schedules.
Synbiotics reduce postoperative infectious complications: a randomized controlled trial in biliary cancer patients undergoing hepatectomy
Synbiotics, combined with early enteral nutrition, can reduce postoperative infections and presumably involves correction of an intestinal microbial imbalance induced by surgical stress.
Clostridium difficile colonization in healthy adults: transient colonization and correlation with enterococcal colonization.
The present results demonstrate that, although colonization by a C. difficile strain is transient in many cases, there are healthy individuals that are colonized persistently by C.difficile, and suggest that dense colonization of the intestine by enterococci may be associated with C. diffusion.
Rapid identification of human intestinal bifidobacteria by 16S rRNA-targeted species- and group-specific primers.
On the basis of 16S rRNA sequences, species- and group-specific primers for Bifidobacterium adolescentis, B. angulatum, B. bifidum, B. breve, the B. catenulatum group (B. catenulatum and B.