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Generation of a nicking enzyme that stimulates site-specific gene conversion from the I-AniI LAGLIDADG homing endonuclease
TLDR
A variant of the I-AniI homing endonuclease that nicks its cognate target site is engineered that contains a mutation of a basic residue essential for proton transfer and solvent activation in one active site. Expand
Biocontainment of genetically modified organisms by synthetic protein design
TLDR
This work computationally redesign essential enzymes in the first organism possessing an altered genetic code to confer metabolic dependence on non-standard amino acids for survival, providing a foundation for safer GMOs that are isolated from natural ecosystems by a reliance on synthetic metabolites. Expand
Tapping natural reservoirs of homing endonucleases for targeted gene modification
TLDR
Biochemical and structural characterization of endonucleases from one clade within the phylogenetic tree demonstrates strong conservation of protein structure contrasted against highly diverged DNA target sites and indicates that a significant fraction of these proteins are sufficiently stable and active to serve as engineering scaffolds. Expand
Purification of Drosophila DNA polymerase zeta by REV1 protein-affinity chromatography.
TLDR
The present study is the first report about characterization of purified Pol zeta from multicellular organisms, and the second concerning the characterization of yeast pol zeta. Expand
Biochemical properties of a plastidial DNA polymerase of rice
TLDR
It is proposed to rename the plant PolI homologs as DNA polymerase π (POLP), and investigate the biochemical properties of full-length OsPOLP1, finding that POLPs have two extra sequences in the polymerase domain that are absent in prokaryotic PolIs. Expand
DmGEN shows a flap endonuclease activity, cleaving the blocked‐flap structure and model replication fork
TLDR
The studies revealed that DmGEN belongs to a new class (class IV) of the RAD2/XPG nuclease family and the biochemical properties of Dm GEN and its possible role are discussed. Expand
Distribution and roles of X-family DNA polymerases in eukaryotes.
TLDR
This review focuses on the interesting pattern of distribution of the X-family enzymes across biological kingdoms and speculate on their roles. Expand
Optimization of in vivo activity of a bifunctional homing endonuclease and maturase reverses evolutionary degradation
TLDR
A selection experiment identified point mutations in the enzyme that act synergistically to improve endonuclease activity that increase target affinity, alter the thermal cleavage profile and significantly increase targeted recombination in transfected cells. Expand
Redesign of extensive protein–DNA interfaces of meganucleases using iterative cycles of in vitro compartmentalization
TLDR
Fusion with transcription activator-like effector DNA binding domains significantly enhances targeted modification induced by meganucleases engineered in this study. Expand
DmGEN, a novel RAD2 family endo-exonuclease from Drosophila melanogaster.
TLDR
In vitro data suggest that DmGEN belongs to a new class of RAD2 nuclease, and locations of the domains are similar to those of FEN-1 and EXO-1, and the molecular weight of the protein is close to that of EXo-1. Expand
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