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A single nucleotide substitution converts potato spindle tuber viroid (PSTVd) from a noninfectious to an infectious RNA for nicotiana tabacum.
TLDR
The sequence analysis of PSTVd NT revealed a single nucleotide substitution from C-->U at position 259, which might be involved in protein binding and in viroid processing in both tobacco and tomato.
Secondary structure probing of potato spindle tuber viroid (PSTVd) and sequence comparison with other small pathogenic RNA replicons provides evidence for central non-canonical base-pairs, large
TLDR
Non-denaturing gel electrophoresis shows that linear (non-circular) unit-length (+)strand RNA molecules of a lethal PSTVd variant were produced which are able to initiate typical disease symptoms when inoculated into tomato.
The molecular structure of Iresine viroid, a new viroid species from Iresine herbstii ('beefsteak plant').
  • R. Spieker
  • Biology
    The Journal of general virology
  • 1 October 1996
TLDR
A viroid was isolated from Iresine herbstii plants using the bidirectional PAGE method for analysis of small circular RNA molecules and is a member of the potato spindle tuber viroid group and consists of 370 nucleotides.
In vitro-generated 'inverse' chimeric Coleus blumei viroids evolve in vivo into infectious RNA replicons.
  • R. Spieker
  • Biology
    The Journal of general virology
  • 1 November 1996
TLDR
The cDNA-transcripts of CbVd A-1 and C bVd-Ch2 are infectious by mechanical inoculation of viroid-free plants of cv.
A viroid fromBrunfelsia undulata closely related to theColumnea latent viroid
TLDR
Cloning and sequencing revealed that the viroid from Brunfelsia undulata is closely related to theColumnea latent viroid (CLVd), therefore, the new viroid sequence variant has been named CLVd-B.
Primary and secondary structure of a new viroid 'species' (CbVd 1) present in the Coleus blumei cultivar 'Bienvenue'
TLDR
The sequence and the secondary structure model of the 248 nt long new viroid, tentatively named Coleus blumei viroid 1 (CbVd 1), are shown in the figure.
A viroid fromSolanum pseudocapsicum closely related to the tomato apical stunt viroid
TLDR
Cloning and sequencing revealed that the viroid from Solanum pseudocapsicum is closely related to the tomato apical stunt viroid (TASVd) and therefore, the new viroid sequence variant has been named TASvd-S.
A new sequence variant ofColeus blumei viroid 1 from theColeus blumei cultivar ‘Rainbow Gold’
TLDR
A viroid was isolated from symptomlessColeus blumei cultivar (cv.) ‘Rainbow Gold’ plants using the bidirectional PAGE method for analysis of small circular RNA molecules and showed a high sequence similarity to the corresponding domain of hop latent viroid (HLVd).
Viroids proper can be distinguished from hammerhead viroids and satellite RNAs through their dinucleotide composition
TLDR
Evaluation of the dinucleotide pattern allows a quick discrimination between viroid proper and similar RNAs from plants, such as hammerhead-containing viroids, satellite RNAs, and defective interfering RNAs.
[The effect of the time of day on the renal excretion of several minerals in cattle].
  • R. Spieker
  • Biology, Medicine
    Berliner und Munchener tierarztliche…
  • 1 February 1989
TLDR
It is shown that excretion of potassium and magnesium doesn't considerably differ at different times of day, but calcium, sodium and inorganic phosphorus show maxima of excretion in the middle of each day and minima at midnight.
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