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- Publications
- Influence
Localization of ASH1 mRNA particles in living yeast.
- E. Bertrand, P. Chartrand, M. Schaefer, S. Shenoy, R. Singer, R. Long
- Medicine, Biology
- Molecular cell
- 1 October 1998
ASH1 mRNA localizes to the bud tip in Saccharomyces cerevisiae to establish asymmetry of HO expression, important for mating type switching. To visualize real time localization of the mRNA in living… Expand
Single-RNA counting reveals alternative modes of gene expression in yeast
- Daniel Zenklusen, D. Larson, R. Singer
- Biology, Medicine
- Nature Structural &Molecular Biology
- 16 November 2008
Proper execution of transcriptional programs is a key requirement of gene expression regulation, demanding accurate control of timing and amplitude. How precisely the transcription machinery fulfills… Expand
In vivo dynamics of RNA polymerase II transcription
- X. Darzacq, Y. Shav-Tal, +4 authors R. Singer
- Medicine, Biology
- Nature Structural &Molecular Biology
- 5 August 2007
We imaged transcription in living cells using a locus-specific reporter system, which allowed precise, single-cell kinetic measurements of promoter binding, initiation and elongation. Photobleaching… Expand
Visualization of single RNA transcripts in situ.
- A. Femino, F. Fay, K. Fogarty, R. Singer
- Biology, Medicine
- Science
- 24 April 1998
Fluorescence in situ hybridization (FISH) and digital imaging microscopy were modified to allow detection of single RNA molecules. Oligodeoxynucleotide probes were synthesized with five fluorochromes… Expand
A general method to improve fluorophores for live-cell and single-molecule microscopy
- J. Grimm, Brian P. English, +9 authors L. Lavis
- Chemistry, Medicine
- Nature Methods
- 27 December 2014
Specific labeling of biomolecules with bright fluorophores is the keystone of fluorescence microscopy. Genetically encoded self-labeling tag proteins can be coupled to synthetic dyes inside living… Expand
Spatial regulation of β-actin translation by Src-dependent phosphorylation of ZBP1
- S. Hüttelmaier, Daniel Zenklusen, +6 authors R. Singer
- Biology, Medicine
- Nature
- 24 November 2005
Localization of β-actin messenger RNA to sites of active actin polymerization modulates cell migration during embryogenesis, differentiation and possibly carcinogenesis. This localization requires… Expand
Sequences responsible for intracellular localization of beta-actin messenger RNA also affect cell phenotype
- E. Kislauskis, X. Zhu, R. Singer
- Biology, Medicine
- The Journal of cell biology
- 2 October 1994
We have characterized the structure and function of RNA sequences that direct beta-cytoplasmic actin mRNA to the cell periphery were mapped to two segments of 3'-untranslated region by expression of… Expand
From Silencing to Gene Expression Real-Time Analysis in Single Cells
- S. Janicki, T. Tsukamoto, +8 authors D. Spector
- Medicine, Biology
- Cell
- 5 March 2004
We have developed an inducible system to visualize gene expression at the levels of DNA, RNA and protein in living cells. The system is composed of a 200 copy transgene array integrated into a… Expand
Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNA.
- R. Long, R. Singer, X. Meng, I. González, K. Nasmyth, R. Jansen
- Biology, Medicine
- Science
- 18 July 1997
Cell divisions that produce progeny differing in their patterns of gene expression are key to the development of multicellular organisms. In the budding yeast Saccharomyces cerevisiae, mother cells… Expand
Characterization of a beta-actin mRNA zipcode-binding protein.
- A. Ross, Y. Oleynikov, E. Kislauskis, K. Taneja, R. Singer
- Biology, Medicine
- Molecular and cellular biology
- 1 April 1997
Localization of beta-actin mRNA to the leading edge of fibroblasts requires the presence of conserved elements in the 3' untranslated region of the mRNA, including a 54-nucleotide element which has… Expand