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Localization of ASH1 mRNA particles in living yeast.
ASH1 mRNA localizes to the bud tip in Saccharomyces cerevisiae to establish asymmetry of HO expression, important for mating type switching. To visualize real time localization of the mRNA in livingExpand
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Single-RNA counting reveals alternative modes of gene expression in yeast
Proper execution of transcriptional programs is a key requirement of gene expression regulation, demanding accurate control of timing and amplitude. How precisely the transcription machinery fulfillsExpand
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Visualization of single RNA transcripts in situ.
Fluorescence in situ hybridization (FISH) and digital imaging microscopy were modified to allow detection of single RNA molecules. Oligodeoxynucleotide probes were synthesized with five fluorochromesExpand
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In vivo dynamics of RNA polymerase II transcription
We imaged transcription in living cells using a locus-specific reporter system, which allowed precise, single-cell kinetic measurements of promoter binding, initiation and elongation. PhotobleachingExpand
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A general method to improve fluorophores for live-cell and single-molecule microscopy
Specific labeling of biomolecules with bright fluorophores is the keystone of fluorescence microscopy. Genetically encoded self-labeling tag proteins can be coupled to synthetic dyes inside livingExpand
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Spatial regulation of β-actin translation by Src-dependent phosphorylation of ZBP1
Localization of β-actin messenger RNA to sites of active actin polymerization modulates cell migration during embryogenesis, differentiation and possibly carcinogenesis. This localization requiresExpand
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Sequences responsible for intracellular localization of beta-actin messenger RNA also affect cell phenotype
We have characterized the structure and function of RNA sequences that direct beta-cytoplasmic actin mRNA to the cell periphery were mapped to two segments of 3'-untranslated region by expression ofExpand
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Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNA.
Cell divisions that produce progeny differing in their patterns of gene expression are key to the development of multicellular organisms. In the budding yeast Saccharomyces cerevisiae, mother cellsExpand
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From Silencing to Gene Expression Real-Time Analysis in Single Cells
We have developed an inducible system to visualize gene expression at the levels of DNA, RNA and protein in living cells. The system is composed of a 200 copy transgene array integrated into aExpand
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Characterization of a beta-actin mRNA zipcode-binding protein.
Localization of beta-actin mRNA to the leading edge of fibroblasts requires the presence of conserved elements in the 3' untranslated region of the mRNA, including a 54-nucleotide element which hasExpand
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