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Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines.
A pilot-scale, in vitro, anticancer drug screen utilizing a panel of 60 human tumor cell lines organized into subpanels representing leukemia, melanoma, and cancers of the lung, colon, kidney, ovary, and central nervous system is described.
The NCI60 human tumour cell line anticancer drug screen
- R. Shoemaker
- Medicine, BiologyNature Reviews. Cancer
- 1 October 2006
The development, use and productivity of the NCI60 screen are reviewed, highlighting several outcomes that have contributed to advances in cancer chemotherapy.
Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines.
The new XTT reagent provides for a simplified, in vitro cell growth assay with possible applicability to a variety of problems in cellular pharmacology and biology, but still shares many of the limitations and potential pitfalls of MTT or other tetrazolium-based assays.
Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.
Since the microculture tetrazolium assay provides sensitive and reproducible indices of growth as well as drug sensitivity in individual cell lines over the course of multiple passages and several months' cultivation, it appears suitable for initial-stage in vitro drug screening.
Discovery of cyanovirin-N, a novel human immunodeficiency virus-inactivating protein that binds viral surface envelope glycoprotein gp120: potential applications to microbicide development
The novel 11-kDa virucidal protein, named cyanovirin-N (CV-N), is isolated and sequenced from cultures of the cyanobacterium (blue-green alga) Nostoc ellipsosporum and is highly resistant to physicochemical denaturation, further enhancing its potential as an anti-HIV microbicide.
Display and analysis of patterns of differential activity of drugs against human tumor cell lines: development of mean graph and COMPARE algorithm.
This study developed a key methodological tool, the mean graph, which allowed the transformation of the numerical cell line response data into graphic patterns that were particularly expressive of differential cell growth inhibition.
Echinomycin, a small-molecule inhibitor of hypoxia-inducible factor-1 DNA-binding activity.
It is shown that it is possible to identify small molecules that inhibit HIF-1 DNA binding to endogenous promoters and echinomycin, a small-molecule known to bind DNA in a sequence-specific fashion, was investigated.
Nelfinavir, A Lead HIV Protease Inhibitor, Is a Broad-Spectrum, Anticancer Agent that Induces Endoplasmic Reticulum Stress, Autophagy, and Apoptosis In vitro and In vivo
Nelfinavir is a lead HIV protease inhibitor with pleiotropic effects in cancer cells and is a Food and Drug Administration–approved drug that could be repositioned as a cancer therapeutic.
New soluble-formazan assay for HIV-1 cytopathic effects: application to high-flux screening of synthetic and natural products for AIDS-antiviral activity.
- O. Weislow, R. Kiser, D. Fine, J. Bader, R. Shoemaker, M. Boyd
- BiologyJournal of the National Cancer Institute
- 19 April 1989
This simple microassay minimizes the number of plate manipulations typically required with other assay methods and, coupled with computerized data collection and analysis, facilitates large-scale screening of agents for potential antiviral activity.
Identification of small molecule inhibitors of hypoxia-inducible factor 1 transcriptional activation pathway.
The luciferase-based high-throughput screen is a feasible tool for the identification of small molecule inhibitors of HIF-1 transcriptional activation and the results suggest that altered Topo-I function may be associated with repression of Hif-1-dependent induction of gene expression.