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Cell-dependent requirement of human immunodeficiency virus type 1 Vif protein for maturation of virus particles
TLDR
A highly sensitive single-round infection assay using a bacterial chloramphenicol acetyltransferase was developed to analyze an early stage of human immunodeficiency virus type 1 replication and concluded that the Vif protein acts through modulation of the Env protein in the virions, directly or indirectly, to enhance viral infectivity in a certain cell type. Expand
Isolation and characterization of a syncytium-inducing, macrophage/T-cell line-tropic human immunodeficiency virus type 1 isolate that readily infects chimpanzee cells in vitro and in vivo
TLDR
HIV-1DH12 tissue culture infections were characterized by extremely rapid replication kinetics, profound cytopathicity, and tropism for chimp and human PBMC, primary human macrophage, and several human T-cell lines. Expand
Administration of an Anti-CD8 Monoclonal Antibody Interferes with the Clearance of Chimeric Simian/Human Immunodeficiency Virus during Primary Infections of Rhesus Macaques
TLDR
These results directly demonstrate that CD8+ T lymphocytes are actively involved in controlling the acute phase of primate lentivirus infections. Expand
Differential glycosylation, virion incorporation, and sensitivity to neutralizing antibodies of human immunodeficiency virus type 1 envelope produced from infected primary T-lymphocyte and macrophage
TLDR
It is demonstrated that HIV-1 released from infected PBMC and MDM cultures differs in its biochemical and antigenic properties. Expand
Neutralizing antibody directed against the HIV–1 envelope glycoprotein can completely block HIV–1/SIV chimeric virus infections of macaque monkeys
TLDR
It is shown that anti–SHIV neutralizing activity, determined in vitro using an assay measuring loss of infectivity, is the absolute requirement for antibody–mediated protection in vivo. Expand
Mutational analysis of the human immunodeficiency virus vpr open reading frame
TLDR
Mutations were introduced by recombinant DNA techniques into the vpr open reading frame of an infectious molecular clone of human immunodeficiency virus type 1 and, when a low-input dose was used, mutants grew significantly more slowly than the wild-type virus. Expand
Human immunodeficiency virus type 1 neutralizing antibodies accelerate clearance of cell–free virions from blood plasma
TLDR
It is shown, by measuring virion RNA, particle–associated p24 Gag protein and virus infectivity, that the clearance of physical and infectious particles from a primary, dual–tropic virus isolate, HIV–1DH12, is very rapid in naive animals, with half–lives ranging from 13 to 26 minutes. Expand
Infection and pathogenicity of chimeric simian-human immunodeficiency viruses in macaques: determinants of high virus loads and CD4 cell killing.
TLDR
The induced immunodeficiency was accompanied by CD4 cell counts of < 50 cells/microL and was associated with Pneumocystis carinii pneumonia, cytomegalovirus meningoencephalitis, lymphoid depletion, and thymic atrophy. Expand
Generation of a chimeric human and simian immunodeficiency virus infectious to monkey peripheral blood mononuclear cells
TLDR
Western immunoblotting analysis of the replicating chimera in PBMC confirmed the hybrid nature of the virus and suggested that the sequence important for macaque cell tropism lies within the LTR, gag, pol, and/or vif sequences of the SIVMAC genome. Expand
Early replication block of human immunodeficiency virus type 1 in monkey cells.
TLDR
The genetic and functional basis of the replication-defective nature of human immunodeficiency virus type 1 (HIV-1) in monkey cells was studied and the sequence encompassing the 3' half of the long terminal repeat, gag and pol genes of HIV-1 was found to be responsible for the growth restriction. Expand
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