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An Improved Protocol for Quantification of Freshwater Actinobacteria by Fluorescence In Situ Hybridization
A combined fixation and permeabilization protocol for CARD-FISH of freshwater samples is developed, confirming that members of this lineage are among the numerically most important Bacteria of freshwater picoplankton.
Microbial Communities in the Surface Mucopolysaccharide Layer and the Black Band Microbial Mat of Black Band-Diseased Siderastrea siderea
Community profiles and species composition associated with two black band-diseased colonies of the coral Siderastrea siderea provide evidence for an association of numerous toxin-producing heterotrophic microorganisms with BBD of corals.
Laboratory studies on adhesion of microalgae to hard substrates
Adhesion of Chlorella vulgaris(chlorophyceae), Nitzschia amphibia(bacillariophceae) and Chroococcus minutus(cyanobacteria) to hydrophobic (perspex, titanium and stainless steel 316-L), hydrophilic
Abundances, Identity, and Growth State of Actinobacteria in Mountain Lakes of Different UV Transparency
Actinobacteria from different subclades of the acI lineage constituted an important autochthonous element of the aquatic microbial communities in many of the studied lakes, potentially also due to their higher UV resistance.
Molecular Detection and Ecological Significance of the Cyanobacterial Genera Geitlerinema and Leptolyngbya in Black Band Disease of Corals
L Laboratory experiments using isolates of BBD Geitlerinema and Leptolyngbya revealed that they could carry out sulfide-resistant oxygenic photosynthesis, a relatively rare characteristic among cyanobacteria, and that they are adapted to the sulfides-rich, low-light BBD environment.
Flow Sorting of Marine Bacterioplankton after Fluorescence In Situ Hybridization
An approach to sort cells from coastal North Sea bacterioplankton by flow cytometry after in situ hybridization with rRNA-targeted horseradish peroxidase-labeled oligonucleotide probes and catalyzed fluorescent reporter deposition illustrates that a combination of CARD-FISH and flow sorting might be a powerful approach to study the diversity and potentially the activity and the genomes of different bacterial populations in aquatic habitats.
Microbial community composition of black band disease on the coral host Siderastrea siderea from three regions of the wider Caribbean
The hypothesis that BBD is composed of variable members of distinct physiological and toxin associated bacterial groups and that the microbial community variation is associated with environmental differences such as anthropogenic impact is supported.
The presence of the cyanobacterial toxin microcystin in black band disease of corals.
Black band disease is a migrating, cyanobacterial dominated, sulfide-rich microbial mat that moves across coral colonies lysing coral tissue, and the first report of the presence of microcystin in a coral disease is reported.
Cyanotoxins from Black Band Disease of Corals and from Other Coral Reef Environments
It was shown that cyanobacterial toxins belonging to the microcystin/nodularin group were produced by cyanob bacteria originating from both BBD and non-BBD sources, and that cyanotoxin production by BBD cyanobacteria can be affected by environmental factors that are present in the microenvironment associated with this coral disease.