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The cornified envelope: a model of cell death in the skin
New insights into the molecular mechanisms and the physiological endpoints of cornification are increasing the understanding of the pathological defects of this unique form of programmed cell death, which is associated with barrier malfunctions and ichthyosis. Expand
Degradation of corneodesmosome proteins by two serine proteases of the kallikrein family, SCTE/KLK5/hK5 and SCCE/KLK7/hK7.
The results strongly suggest that the two kalikreins are involved in desquamation, a model that could be regulated by a precisely controlled protease-protease inhibitor balance. Expand
Refined Characterization of Corneodesmosin Proteolysis during Terminal Differentiation of Human Epidermis and Its Relationship to Desquamation*
Immunoblotting, immunohistochemistry, and immunoelectron microscopy experiments showed that the first step in corneodesmosin processing is the cleavage of its extremities and probably occurs before its incorporation into desmosomes. Expand
Identification and characterization of a novel retroviral-like aspartic protease specifically expressed in human epidermis.
Indinavir, a potent HIV protease inhibitor used in AIDS therapy, had a significant inhibitory effect on rSASPase auto-activation, which could explain its side effects on skin. Expand
Identification and Cloning of a New Calmodulin-like Protein from Human Epidermis*
After separating by two-dimensional gel electrophoresis an extract of total proteins from human stratum corneum, two spots were extracted and analyzed for their peptide sequence. The resultingExpand
Morphological and biochemical characterization of the cornified envelopes from human epidermal keratinocytes of different origin.
No difference could be detected in the peptide maps of CEf and CEr obtained after tape stripping from the same healthy volunteer indicating that CE maturation within the stratum corneum does not involve the provision of qualitatively new proteins. Expand
A constitutive NADPH oxidase-like system containing gp91phox homologs in human keratinocytes.
Keratinocytes expressed a Nox distinct from the phox isoform of phagocytes providing molecular evidence for a source of superoxide that may regulate cell proliferation and host defense in skin and oral mucosa. Expand
Analysis of proteins with caseinolytic activity in a human stratum corneum extract revealed a yet unidentified cysteine protease and identified the so-called "stratum corneum thiol protease" as
The results indicate that the stratum corneum thiol protease is probably expressed as a pro-enzyme in the lower layers of the epidermis and in part activated by a yet unidentified mechanism in the upper layers during keratinocyte differentiation, in contrast to earlier reports. Expand