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Crystal structure of glucose oxidase from Aspergillus niger refined at 2.3 A resolution.
Glucose oxidase (beta-D-glucose: oxygen 1-oxidoreductase, EC is an FAD-dependent enzyme that catalyzes the oxidation of beta-D-glucose by molecular oxygen. The crystal structure of theExpand
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Purification and characterization of a NADH oxidase from the thermophile Thermus thermophilus HB8.
A NADH oxidase has been purified from the extreme thermophile Thermus thermophilus HB8 by several chromatographic steps. The purified enzyme was essentially homogeneous as judged by gelExpand
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Cloning and characterization of a new laccase from Bacillus licheniformis catalyzing dimerization of phenolic acids
A new laccase gene (cotA) was cloned from Bacillus licheniformis and expressed in Escherichia coli. The recombinant protein CotA was purified and showed spectroscopic properties, typical for blueExpand
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Anatomy of lipase binding sites: the scissile fatty acid binding site.
Shape and physico-chemical properties of the scissile fatty acid binding sites of six lipases and two serine esterases were analyzed and compared in order to understand the molecular basis ofExpand
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Comparative study of methods for extraction and purification of environmental DNA from soil and sludge samples
An important prerequisite for successful construction of a metagenome library is an efficient procedure for extracting DNA from environmental samples. We compared three indirect and four directExpand
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Extracellular lipase of Pseudomonas sp. strain ATCC 21808: purification, characterization, crystallization, and preliminary X-ray diffraction data.
A procedure for the purification of a very hydrophobic lipase from Pseudomonas sp. strain ATCC 21808 was elaborated by avoiding the use of long-chain detergents in view of subsequent crystallizationExpand
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A continuous spectrophotometric assay for P450 BM-3, a fatty acid hydroxylating enzyme, and its mutant F87A.
Cytochrome P450 BM-3 from Bacillus megaterium catalyzes the subterminal hydroxylation of medium- and long-chain fatty acids at the positions omega-1, omega-2, and omega-3. A rapid and continuousExpand
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Crystal structure of NADH oxidase from Thermus thermophilus
The crystal structures of the flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) containing isoforms of NADH oxidase from Thermus thermophilus have been determined by isomorphous andExpand
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The Laccase Engineering Database: a classification and analysis system for laccases and related multicopper oxidases
The Laccase Engineering Database (LccED, http://www.lcced.uni-stuttgart.de) was designed to serve as a tool for a systematic sequence-based classification and analysis of the diverse multicopper oxidase protein family. Expand
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Screening, purification and properties of a thermophilic lipase from Bacillus thermocatenulatus.
By screening of 15 thermophilic Bacillus strains, five strains exhibiting lipase activity were found. Among these the strain Bacillus thermocatenulatus (DSM 730) produced the highest lipase activity.Expand
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