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Production of the antimalarial drug precursor artemisinic acid in engineered yeast
The engineering of Saccharomyces cerevisiae to produce high titres (up to 100 mg l-1) of artemisinic acid using an engineered mevalonate pathway, amorphadiene synthase, and a novel cytochrome P450 monooxygenase from A. annua that performs a three-step oxidation of amorpha-4,11-diene to art Artemisinic acid.
Neonicotinoid insecticides induce salicylate-associated plant defense responses
- Kevin A. Ford, J. Casida, M. Wildermuth
- BiologyProceedings of the National Academy of Sciences
- 27 September 2010
Using Arabidopsis thaliana, it is shown that the neonicotinoid compounds, imidacloprid (IMI) and clothianidin (CLO), via their 6-chloropyridinyl-3-carboxylic acid and 2-chlorothiazolyl-5-car boxylic Acid metabolites, respectively, induce salicylic acid (SA)-associated plant responses associated with enhanced stress tolerance.
Function and Structure of MalA/MalA', Iterative Halogenases for Late-Stage C-H Functionalization of Indole Alkaloids.
- A. Fraley, M. Garcia‐Borràs, D. Sherman
- Chemistry, BiologyJournal of the American Chemical Society
- 21 August 2017
Structural analysis and computational studies of MalA' in complex with three substrates revealed that the enzyme represents a new class of zinc-binding flavin-dependent halogenases and provides new insights into a potentially unique reaction mechanism.
Heathcock-inspired strategies for the synthesis of fawcettimine-type Lycopodium alkaloids.
A number of recent Heathcock-inspired syntheses of fawcettimine-type Lycopodium alkaloids are explored, including one of the most efficient strategies, and alternative, novel disconnections are discussed.
A Unifying Synthesis Approach to the C18-, C19-, and C20-Diterpenoid Alkaloids.
The efforts to prepare these molecules by chemical synthesis, including a photochemical approach, and ultimately a bioinspired strategy that has resulted in the development of a unifying synthesis of one C18 (weisaconitine D), one C19 (liljestrandinine), and three C20 natural products from a common intermediate.
Remarkable facilitation of hetero-cycloisomerizations with water and other polar protic solvents: metal-free synthesis of indolizines.
- Alison R Hardin Narayan, R. Sarpong
- ChemistryGreen chemistry : an international journal and…
- 3 September 2010
Hetero-cycloisomerization reactions of propargylic alcohol derivatives leading to indolizines have been demonstrated to proceed in the presence of water alone. This stands as a significant advance…
Atropurpuran - Missing Biosynthetic Link Leading to the Hetidine and Arcutine C20-Diterpenoid Alkaloids or an Oxidative Degradation Product?
Surveying ubiquitin structure by noncovalent attachment of distance constrained bis(crown) ethers.
- T. Ly, Zhenjiu Liu, B. G. Pujanauski, R. Sarpong, R. Julian
- Biology, ChemistryAnalytical chemistry
- 23 May 2008
PBC is shown to be an effective probe of protein structure in SNAPP experiments, although assigning the specific residues to which PBC is attached can be experimentally challenging.
S-Arachidonoyl-2-thioglycerol synthesis and use for fluorimetric and colorimetric assays of monoacylglycerol lipase.