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Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified. Expand
Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.
Two new methods were used to establish a rapid and highly sensitive prenatal diagnostic test for sickle cell anemia. The first involves the primer-mediated enzymatic amplification of specific… Expand
Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction.
- K. Mullis, F. Faloona, S. Scharf, R. Saiki, G. Horn, H. Erlich
- Chemistry, Medicine
- Cold Spring Harbor symposia on quantitative…
An alternative method for the synthesis of specific DNA sequences is explored that involves the reciprocal interaction of two oligonucleotides and the DNA polymerase extension products whose synthesis they prime, when they are hybridized to different strands of a DNA template in a relative orientation such that their extension products overlap. Expand
A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions.
These procedures, which can circumvent the need for large-scale phage or plasmid growths, preparative gel-electrophoresis and the screening of molecular clones, can facilitate the rapid study of sequence-specific interactions of proteins and DNA. Expand
Enzymatic amplification of ?-globin genomic sequences and restriction site analysis for diagnosis of
ExoSAP-IT offers quick, one step PCR clean-up with 100% recovery of both short and long PCR products, and is completely scaleable for manual or automated use. Expand
A missense single-nucleotide polymorphism in a gene encoding a protein tyrosine phosphatase (PTPN22) is associated with rheumatoid arthritis.
- A. Begovich, V. Carlton, +25 authors P. Gregersen
- Biology, Medicine
- American journal of human genetics
- 1 August 2004
It is shown that the risk allele of a missense SNP in PTPN22 disrupts the P1 proline-rich motif that is important for interaction with Csk, potentially altering these proteins' normal function as negative regulators of T-cell activation. Expand
Analysis of enzymatically amplified β-globin and HLA-DQα DNA with allele-specific oligonucleotide probes
The polymerase chain reaction (PCR) procedure is used to enzymatically amplify a specific segment of the β-globin or HLA-DQα gene in human genomic DNA before hybridization with ASOs, enabling the analysis of allelic variation with as little as 1 ng of genomic DNA and the use of a simple ‘dot blot’ for probe hybridization. Expand
Genetic analysis of amplified DNA with immobilized sequence-specific oligonucleotide probes.
- R. Saiki, P. Walsh, C. Levenson, H. Erlich
- Biology, Medicine
- Proceedings of the National Academy of Sciences…
- 1 August 1989
A method by which one can simultaneously screen a sample for all known allelic variants at an amplified locus is described, applied to HLA-DQA genotyping and to the detection of Mediterranean beta-thalassemia mutations. Expand
The Design and Optimization of the PCR
- R. Saiki
In the few years since its introduction,1,2,3 the polymerase chain reaction has already become a widespread research technique. Like the PCR itself, the numbers of its practitioners have been… Expand
Analysis of enzymatically amplified beta-globin and HLA-DQ alpha DNA with allele-specific oligonucleotide probes.
The polymerase chain reaction (PCR) procedure is used to enzymatically amplify a specific segment of the beta-globin or HLA-DQ alpha gene in human genomic DNA before hybridization with ASOs, enabling the analysis of allelic variation with as little as 1 ng of genomic DNA and the use of a simple 'dot blot' for probe hybridization. Expand