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Macronuclear Genome Sequence of the Ciliate Tetrahymena thermophila, a Model Eukaryote
The ciliate Tetrahymena thermophila is a model organism for molecular and cellular biology. Like other ciliates, this species has separate germline and soma functions that are embodied by distinct…
Methylation of Histone H3 at Lysine 9 Targets Programmed DNA Elimination in Tetrahymena
Parental expression of the chromodomain protein Pdd1p is required for completion of programmed DNA elimination and nuclear differentiation.
A leucine zipper domain of the suppressor of Hairy-wing protein mediates its repressive effect on enhancer function.
A region of su(Hw) homologous to the leucine zipper motif is necessary for the negative effect of this protein on enhancer function, suggesting that su( Hw) might exert this effect by interacting, directly or indirectly, with transcription factors bound to these enhancers.
The Drosophila su(Hw) gene, which controls the phenotypic effect of the gypsy transposable element, encodes a putative DNA-binding protein.
A transcript encodes a protein that contains a highly acidic region and 12 repeats of the 'Zn finger' domain characteristic of some DNA-binding and transcription-activating proteins, supporting the hypothesis that the su(Hw) locus might encode a transcription factor that plays a role in the expression of the gypsy element.
Pdd1p, A Novel Chromodomain-Containing Protein, Links Heterochromatin Assembly and DNA Elimination in Tetrahymena
Genome downsizing during ciliate development: nuclear division of labor through chromosome restructuring.
It is postulate that ciliate nuclear restructuring serves to segregate the two essential functions of chromosomes: the transmission and expression of genetic information.
Cell shape and interaction defects in alpha-spectrin mutants of Drosophila melanogaster
- J. K. Lee, R. S. Coyne, R. Dubreuil, L. Goldstein, D. Branton
- BiologyThe Journal of cell biology
- 2 December 1993
Examination of spectrin deficient larvae suggests that an important role of non-erythroid spectrin is to stabilize cell to cell interactions that are critical for the maintenance of cell shape and subcellular organization within tissues.
Genome-Scale Analysis of Programmed DNA Elimination Sites in Tetrahymena thermophila
Results from the first high-throughput sequencing effort to investigate ciliate genome restructuring are described, comparing Sanger long-read sequences from a Tetrahymena thermophila MIC genome library to the MAC genome assembly and discovering a previously unknown class of small IES with precise elimination boundaries that can contribute the 3′ exon of an mRNA expressed during genome restructuring.
Identification of novel chromatin-associated proteins involved in programmed genome rearrangements in Tetrahymena
It is found that late in macronuclear differentiation, four of the newly identified proteins are organized into nuclear foci that also contain Pdd1p, a result that further implicates this structure in the regulation of DNA rearrangement.