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Integration of DNA barcoding into an ongoing inventory of complex tropical biodiversity
- D. Janzen, W. Hallwachs, John-James Wilson
- Biology, Environmental ScienceMolecular ecology resources
- 1 May 2009
Adding DNA barcoding to the inventory of the caterpillars, their food plants and parasitoids in northwestern Costa Rica has substantially improved the quality and depth of the inventory, and greatly multiplied the number of situations requiring further taxonomic work for resolution.
DNA Barcoding Reveals Cryptic Diversity in Lumbricus terrestris L., 1758 (Clitellata): Resurrection of L. herculeus (Savigny, 1826)
Large numbers of DNA barcode sequences of the cytochrome oxidase I obtained for nominal L. terrestris and six congeneric species reveal that there are two distinct lineages within nominal L, herculeus, and the two are morphologically indistinguishable, except by overlapping size-related characters.
Wolbachia and DNA Barcoding Insects: Patterns, Potential, and Problems
It is concluded that regular assays for Wolbachia presence and type can, and should, be adopted by large scale insect barcoding initiatives and the ability to query this DNA library for endosymbionts is one of the ancillary benefits of such a large scale endeavor.
Species-Level Para- and Polyphyly in DNA Barcode Gene Trees: Strong Operational Bias in European Lepidoptera
It is suggested that species-level non-monophyly in COI gene trees is less common than previously supposed, with many cases reflecting misidentifications, the subjectivity of species delimitation or other operational factors.
Mitochondrial and microsatellite DNA markers reveal a Balkan origin for the highly invasive horse‐chestnut leaf miner Cameraria ohridella (Lepidoptera, Gracillariidae)
Findings suggest that European populations of C. ohridella may indeed derive from the southern Balkans, and high haplotype diversity and low measures of nucleotide diversities including a significantly negative Tajima’s D indicate that C. Ohridella has experienced rapid population expansion during its dispersal across Europe.
Genetic Patterns in European Geometrid Moths Revealed by the Barcode Index Number (BIN) System
This study confirms the effectiveness of DNA barcoding as a tool for species identification and illustrates the potential of the BIN system to characterize formal genetic units independently of an existing classification, and suggests the system can be used to efficiently assess the biodiversity of large, poorly known assemblages of organisms.
A global checklist of the Bombycoidea (Insecta: Lepidoptera)
A global checklist of Bombycoidea, the first effort to synthesize the current taxonomic treatment of the entire superfamily, is presented, accounting for the recent burst in species and subspecies descriptions within family Saturniidae and to a lesser extent in Sphingidae.
Identifying earthworms through DNA barcodes: Pitfalls and promise
Coupling non‐destructive DNA extraction and voucher retrieval for small soft‐bodied Arthropods in a high‐throughput context: the example of Collembola
The lysis step substantially shortens the clearing process necessary for morphological examination, allowing voucher specimen recovery for Collembola and by extension for other soft‐bodied small arthropods.
Morphology and DNA barcoding reveal three cryptic species within the Xylophanes neoptolemus and loelia species-groups (Lepidoptera: Sphingidae)
Three new species are described within the genus Xylophanes, of which the first is known only from Guerrero and Michoacan states in Mexico while the second is widely distributed in lowland forests of Central America and the third is relatives of X. neoptolemus.