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Characterization of two forms of asparaginase in Saccharomyces cerevisiae.
Saccharomyces cerevisiae X2180-1A synthesizes two forms of asparaginase: L-asparaginase I, an internal constitutive enzyme, and asparaginase II, an external enzyme which is secreted in response toExpand
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Asparaginase II of Saccharomyces cerevisiae. Characterization of the ASP3 gene.
Purified preparations of asparaginase II of Saccharomyces cerevisiae exhibit two protein bands upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Cloning and sequencing of the ASP3 gene,Expand
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Methylamine and ammonia transport in Saccharomyces cerevisiae.
Methylamine (methylammonium ion) entered Saccharomyces cerevisiae X2180-A by means of a specific active transport system. Methylamine uptake was pH dependent (maximum rate between pH 6.0 and 6.5) andExpand
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Urea amidolyase. I. Properties of the enzyme from Candida utilis.
Abstract Candida utilis contains an inducible enzyme, urea amidolyase, which catalyzes the decomposition of urea with formation of carbon dioxide and ammonia. This reaction is accompanied by theExpand
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L-Quisqualic acid transport into hippocampal neurons by a cystine-sensitive carrier is required for the induction of quisqualate sensitization
A brief exposure of hippocampal slices to L-quisqualic acid sensitizes CA1 pyramidal neurons 30-250-fold to depolarization by two classes of excitatory amino acid analogues: (1) those whoseExpand
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Possible site-specific reagent for the general amino acid transport system of Saccharomyces cerevisiae.
The general amino acid transport system of Saccharomyces cerevisiae functions in the uptake of neutral, basic, and acidic amino acids. The amino acid analogue N-delta-chloroacetyl-L-ornithine (NCAO)Expand
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Regulation of the nicotinamide adenine dinucleotide- and nicotinamide adenine dinucleotide phosphate-dependent glutamate dehydrogenases of Saccharomyces cerevisiae.
Saccharomyces cerevisiae contains two distinct l-glutamate dehydrogenases. These enzymes are affected in a reciprocal fashion by growth on ammonia or dicarboxylic amino acids as the nitrogen source.Expand
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Urea amidolyase. The involvement of biotin in urea cleavage.
Abstract The ATP-dependent cleavage of urea, catalyzed by urea amidolyase, occurs in two steps: (a) urea + ATP + HCO3- (Mg++, K+)/⇌ allophanate + ADP + Pi; and (b) allophanate → 2 HCO3- + 2 NH4+. TheExpand
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Inhibition of amino acid transport by ammonium ion in Saccharomyces cerevisiae.
The rate of transport of L-amino acids by Saccharomyces cerevisiae epsilon 1278b increased with time in response to nitrogen starvation. This increase could be prevented by the addition of ammoniumExpand
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Nitrogen catabolite repression in a glutamate auxotroph of Saccharomyces cerevisiae.
The biosynthesis of asparaginase II in Saccharomyces cerevisiae is subject to nitrogen catabolite repression. In the present study we examined the physiological effects of glutamate auxotrophy onExpand
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