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Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
TLDR
A procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters, including tRNA and Ad 2 VA, is developed.
Regulation of MLL1 H3K4 methyltransferase activity by its core components
TLDR
This study reports the first biochemical reconstitution of a functional four-component mixed-lineage leukemia protein-1 (MLL1) core complex and demonstrates that WDR5 mediates interactions of the MLL1 catalytic unit both with the common structural platform and with the histone substrate.
Human PAD4 Regulates Histone Arginine Methylation Levels via Demethylimination
TLDR
It is demonstrated that human peptidylarginine deiminase 4 (PAD4) regulates histone Arg methylation by converting methyl-Arg to citrulline and releasing methylamine, and that PAD4 activity is linked with the transcriptional regulation of estrogen-responsive genes in MCF-7 cells.
Crystal structure of a TFIIB–TBP–TATA-element ternary complex
The crystal structure of the transcription factorIIB (TFIIB)/TATA box-binding protein (TBP)/ TATA-element ternary complex is described at 2.7 Å resolution. Core TFIIB resembles cyclin A, and
The metazoan Mediator co-activator complex as an integrative hub for transcriptional regulation
TLDR
The Mediator is an evolutionarily conserved, multiprotein complex that is a key regulator of protein-coding genes in metazoan cells and can interact with and coordinate the action of numerous other co-activators and co-repressors, including those acting at the level of chromatin.
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