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The effect of the human serum paraoxonase polymorphism is reversed with diazoxon, soman and sarin
TLDR
A simple enzyme analysis is reported that provides a clear resolution of PON1 genotypes and phenotypes allowing for a reasonable assessment of an individual's probable susceptibility or resistance to a given OR, extending earlier studies on this system. Expand
Paraoxonase (PON1) Phenotype Is a Better Predictor of Vascular Disease Than Is PON1192 or PON155 Genotype
TLDR
The results support the benefit of a “level crossing” approach that includes intervening phenotypes in the study of complexly inherited disease. Expand
Polymorphisms in the human paraoxonase (PON1) promoter.
TLDR
A deletion analysis of the PON1 promoter region in transient transfection assays found that cell-type specific promoter elements for liver and kidney are present in the first 200bp upstream of the coding sequence. Expand
Catalytic efficiency determines the in-vivo efficacy of PON1 for detoxifying organophosphorus compounds.
TLDR
Kinetic analysis of the two human PON1(192) isoforms revealed that the catalytic efficiency (Vmax/Km) determines the in-vivo efficacy of Pon1 for organophosphorus detoxication. Expand
The role of paraoxonase (PON1) in the detoxication of organophosphates and its human polymorphism.
TLDR
The experiments with a mouse model system have convincingly shown that PON1 plays a major role in the detoxication of organophosphate (OP) compounds processed through the P450/PON1 pathway and the high level of variation of gene expression within each genetic class in humans, together with animal model studies indicate that it is very important to determine PON status as opposed to Pon1 genotype alone. Expand
Determination of paraoxonase (PON1) status requires more than genotyping.
TLDR
A two-dimensional, microtitre plate reader-based enzyme analysis that provides a high-throughput assessment of PON1 status and an accurate inference of Pon1 genotype is reported here. Expand
Spectrophotometric assays for the enzymatic hydrolysis of the active metabolites of chlorpyrifos and parathion by plasma paraoxonase/arylesterase.
TLDR
A greater than 40-fold difference in rates of chlorpyrifosoxon hydrolysis observed between rat (low activity) and rabbit sera (high activity) correlated well with the reported large differences in LD50 values for chlorp Pyrifos in these two animals, consistent with an important role of serum paraoxonase in detoxification of organophosphorus pesticides in vivo. Expand
Characterization of cDNA clones encoding rabbit and human serum paraoxonase: the mature protein retains its signal sequence.
TLDR
The rabbit and human cDNA clones demonstrate striking nucleotide and deduced amino acid similarities (greater than 85%), suggesting an important metabolic role and constraints on the evolution of this protein. Expand
Paraoxonase (PON 1) as a biomarker of susceptibility for organophosphate toxicity
TLDR
In vitro assayed catalytic efficiencies of purified PON192 isoforms for hydrolysis of specific oxon substrates accurately predict the degree of in vivo protection afforded by each isoform, and may contribute to the higher sensitivity of newborns to OP toxicity. Expand
Paraoxonase Polymorphisms, Haplotypes, and Enzyme Activity in Latino Mothers and Newborns
TLDR
Most of the newborn children and some pregnant women in this Latino cohort may have elevated susceptibility to organophosphate toxicity because of their PON1192 genotype and low PON1 plasma levels. Expand
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