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Molecular origin of cancer: catechol estrogen-3,4-quinones as endogenous tumor initiators.
The hypothesis that CE-3,4-Q are endogenous tumor initiators is supported, supported by data that indicates that depurinating hydrocarbon-DNA adducts generate oncogenic mutations found in mouse skin papillomas. Expand
Metabolism and DNA binding studies of 4-hydroxyestradiol and estradiol-3,4-quinone in vitro and in female ACI rat mammary gland in vivo.
Results demonstrate that the 4-CE are metabolized to CE-3,4-Q, which react with DNA to form primarily depurinating adducts, which can generate the critical mutations that initiate cancer. Expand
Metabolism of loratadine and further characterization of its in vitro metabolites.
The present study demonstrated that in addition to CYP3A4 and CYP2D6, the metabolism of loratadine is also catalyzed by CYP1A1, CYP2C19, and to a lesser extent by CYP1A2, CYP2B6, CYP2C8, CYP2C9 andExpand
It is time for a paradigm shift in drug discovery bioanalysis: from SRM to HRMS.
Some insight is provided into the evolution of the hybrid quadrupole-time-of-flight (Qq-TOF) mass spectrometer and some of the desirable specifications that such HRMS systems should have to be integrated into the drug discovery bioanalytical workflow for performing integrated qualitative and quantitative bioanalysis of drugs and related components are proposed. Expand
Response normalized liquid chromatography nanospray ionization mass spectrometry
The data obtained from four different structural classes of compounds and their metabolites indicate that by maintaining the solvent composition unchanged across the HPLC run, the influence of the solvent environment on the ionization efficiency is minimized. Expand
Synthesis and structure elucidation of estrogen quinones conjugated with cysteine, N-acetylcysteine, and glutathione.
The chemical synthesis and characterization of these conjugates are the first essential steps to better understand their function in biological systems and will be used as standards in the identification of these compounds formed in biological Systems. Expand
Detection and quantification of depurinated benzo[a]pyrene-adducted DNA bases in the urine of cigarette smokers and women exposed to household coal smoke.
Urine samples were fractionated by a combination of SepPak extraction and reverse-phase HPLC, and analyzed by tandem mass spectrometry and capillary electrophoresis with laser-induced fluorescence to justify more extensive studies of depurinated BP-adducted DNA bases as potential biomarkers of PAH-associated cancer risk. Expand
Investigation of calcium-induced, noncovalent association of calmodulin with melittin by electrospray ionization mass spectrometry
Noncovalent association of Ca2+-loaded calmodulin with a target peptide melittin was studied by electrospray ionization mass spectrometry (ESI-MS). ESI-MS does not reveal any binding of theExpand
Identification of Human Liver Cytochrome P450 Enzymes Involved in the Metabolism of SCH 530348 (Vorapaxar), a Potent Oral Thrombin Protease-Activated Receptor 1 Antagonist
The results suggest that CYP3A4 and CYP2J2 are both involved in the formation of M20, a major circulating human metabolite at steady state, which is pharmacologically equipotent to SCH 530348, whereas M19 is an inactive metabolite. Expand
Quantitative determination of hippuric and benzoic acids in urine by LC-MS/MS using surrogate standards.
The proposed LC-MS/MS method was successfully utilized for analysis of urine samples from female monkeys following the administration of everninomicin alone and in combination with gentamicin, and was assessed by analyzing 3 quality control samples prepared in monkey urine. Expand