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CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex
A conserved γ-tubulin complex–binding domain in CDK5RAP2 stimulates the microtubule-nucleating activity of γ-TuRC.
CDK5RAP2 is a pericentriolar protein that functions in centrosomal attachment of the gamma-tubulin ring complex.
Together, CDK5RAP2 is a pericentriolar structural component that functions in gammaTuRC attachment and therefore in the microtubule organizing function of the centrosome, suggesting that centrosomes malfunction due to the CDK 5R AP2 mutations may underlie autosomal recessive primary microcephaly. Expand
α2-Chimaerin, Cyclin-Dependent Kinase 5/p35, and Its Target Collapsin Response Mediator Protein-2 Are Essential Components in Semaphorin 3A-Induced Growth-Cone Collapse
Results indicate that active α2-chimaerin Rac GAP, Cdk5/p35, and its substrate CRMP-2, are implicated in the dynamics of growth cone guidance initiated through Sema 3A signaling. Expand
Conserved Motif of CDK5RAP2 Mediates Its Localization to Centrosomes and the Golgi Complex*
  • Zhe Wang, Tao Wu, +5 authors R. Qi
  • Biology, Medicine
  • The Journal of Biological Chemistry
  • 13 May 2010
It is demonstrated that the CM2-like motif plays a crucial role in the centrosomal and Golgi localization of CDK5RAP2, a γ-tubulin complex-binding protein that contains a centrosome-targeting domain highly homologous to the Motif 2 of centrosomin, a functionally related protein in Drosophila. Expand
Molecular Pathway of Microtubule Organization at the Golgi Apparatus.
CAMSAP2 stabilizes and attaches microtubule minus ends to the Golgi through a complex of AKAP450 and myomegalin, and proposes that Golgi-anchored microtubules are important for polarized cell movement but not for coalescence of Golgi membranes. Expand
Interaction of CDK5RAP2 with EB1 to track growing microtubule tips and to regulate microtubule dynamics.
Results show that CDK5RAP2 targets growing microtubule tips in association with EB1 to regulate microtubules dynamics and stability and depletion by RNA interference impacts the dynamic behaviors of micro Tubules. Expand
Specific inhibition of cyclin-dependent kinases and cell proliferation by harmine.
In cytotoxicity assays, harmine exhibited a strong inhibitory effect on the growth and proliferation of carcinoma cells whereas it had no significant effect on quiescent fibroblasts, and taken together, harmsine is a selective inhibitor of CDKs and cell proliferation. Expand
A Combined Proteomics and Metabolomics Profiling of Gastric Cardia Cancer Reveals Characteristic Dysregulations in Glucose Metabolism*
Using proteomics and metabolomics approaches, it is found that the level of several enzymes and their related metabolic intermediates involved in glucose metabolism were deregulated in GCC, providing potential therapeutic targets for this disease. Expand
The Protein SET Binds the Neuronal Cdk5 Activator p35 nck5a and Modulates Cdk5/p35 nck5a Activity*
The detailed characterization showed that the SET protein formed a complex with Cdk5/p35 nck5a through its binding to p35 ncks5a and the tail region of SET, which is rich in acidic residues, is required for the stimulatory effect on Cdk 5/p 35 nck 5a. Expand
Direct Regulation of Microtubule Dynamics by Protein Kinase CK2*
CK2 is a microtubule-associated protein that confers micro Tubule stability in a phosphorylation-independent manner and over-expression of chicken CK2α or its kinaseinactive mutant in the endogenous CK2 α/α′-depleted cells fully restored the microtubules resistance to the low dose of colchicine. Expand