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Cyclin A in cell cycle control and cancer
Consistent with its role as a key cell cycle regulator, expression of cyclin A is found to be elevated in a variety of tumors.
Regulation of the CDK-related protein kinase PCTAIRE-1 and its possible role in neurite outgrowth in Neuro-2A cells.
- R. Graeser, J. Gannon, R. Poon, T. Dubois, A. Aitken, T. Hunt
- BiologyJournal of cell science
- 1 September 2002
It is found that various forms of PCTAIRE-1 transfected into neuroblastoma cell lines could either promote or inhibit neurite outgrowth, suggesting a potential role for the PCTAFE-1 gene product in the control of neuriteOutgrowth.
Dephosphorylation of Cdk2 Thr160 by the Cyclin-Dependent Kinase-Interacting Phosphatase KAP in the Absence of Cyclin
Results suggest that KAP binds to Cdk2 and dephosphorylates Thr160 when the associated cyclin subunit is degraded or dissociates, and prevented Cdk1 kinase activity upon subsequent association with cyclin A.
The multiple checkpoint functions of CHK1 and CHK2 in maintenance of genome stability.
An overview of how the multi-tasking nature of CHK1 and CHK2 is achieved in vertebrate cells is provided.
Cyclin-dependent Kinases Are Inactivated by a Combination of p21 and Thr-14/Tyr-15 Phosphorylation after UV-induced DNA Damage*
- R. Poon, W. Jiang, H. Toyoshima, T. Hunter
- Biology, ChemistryThe Journal of Biological Chemistry
- 31 May 1996
Investigation of the contribution of p21 in inhibiting different cyclin-CDK complexes that drive different cell cycle transitions following UV irradiation-induced DNA damage in normal human fibroblasts and immortalized rodent fibro Blasts suggests that different CDKs are inhibited by different mechanisms following UV-inducedDNA damage.
A roller coaster ride with the mitotic cyclins.
Redistribution of the CDK inhibitor p27 between different cyclin.CDK complexes in the mouse fibroblast cell cycle and in cells arrested with lovastatin or ultraviolet irradiation.
It is found that the level of p27 is elevated in cells arrested in G0 by growth factor deprivation or contact inhibition, and the loss of the monomeric p27 pool as cyclins accumulate in G1 is consistent with the in vivo and in vitro data showing that p27 binds better to cyclin.
Cyclin-Dependent Kinases and S Phase Control in Mammalian Cells
The current understanding of the mechanism of initiation of DNA replication in mammalian cells is summarized, and the roles of cyclin A/E-CDK2 complexes in driving replication, their relationship with other regulators of S phase, and their role in keeping replication to only once per cell cycle will be discussed.
Synchronization of HeLa Cells.
Several protocols for synchronizing HeLa cells from different phases of the cell cycle are described, including G1 phase using the HMG-CoA reductase inhibitor lovastatin, S phase with a double thymidine block procedure, and G2 phase with the CDK1 inhibitor RO-3306.
Identification of Functional Domains in the Neuronal Cdk5 Activator Protein*
Three distinct regions in p35 are identified required for binding to Cdk5 or activation of Cdk 5 in vitro, and residues ∼279-291 were needed in addition for activation ofCdk5 in vitro.