Skip to search formSkip to main contentSkip to account menu
Hydroxylation of chlorzoxazone as a specific probe for human liver cytochrome P-450IIE1.
TLDR
Results provide strong evidence that P-450IIE1 is the primary catalyst of chlorzoxazone 6-hydroxylation in human liver and may have potential use as a noninvasive probe in estimating the in vivo expression of the protein.
View on PubMed
Mechanism-based inactivation of human liver cytochrome P450 2A6 by 8-methoxypsoralen.
TLDR
Potency and selectivity suggest that 8-MOP could be a useful tool in vitro for evaluating P450 2A6 activity in various enzyme preparations.
View on PubMed
Hydroxylation of chlorzoxazone as a specific probe for human liver cytochrome P-450IIE1
TLDR
Results provide strong evidence that P-450IIE1 is the primary catalyst of chlorzoxazone 6-hydroxylation in human liver and may have potential use as a noninvasive probe in estimating the in vivo expression of the protein.
View via Publisher
Isoform specificity of trimethylamine N-oxygenation by human flavin-containing monooxygenase (FMO) and P450 enzymes: selective catalysis by FMO3.
View on PubMed
Oxidation of acetaminophen to its toxic quinone imine and nontoxic catechol metabolites by baculovirus-expressed and purified human cytochromes P450 2E1 and 2A6.
TLDR
Although at toxic doses of APAP P 450 2E1 is the more efficient catalyst for the formation of the toxic metabolite NAPQI, P450 2A6 also can contribute significantly to NAPZI production.
View on PubMed
(R)-(+)-Menthofuran is a potent, mechanism-based inactivator of human liver cytochrome P450 2A6.
TLDR
Addition of various nucleophiles, a chelator of iron, or scavengers of reactive oxygen species or extensive dialysis failed to protect CYP2A6 from inactivation, and menthofuran caused a time- and concentration-dependent loss of CYP 2A6 activity.
View on PubMed
Human cytochrome P450 2E1 is a major autoantigen associated with halothane hepatitis.
TLDR
Patients diagnosed with halothane hepatitis have autoantibodies that react with human cytochrome P450 2E1 that was purified from a baculovirus expression system and appeared to be directed against mainly conformational epitopes.
View on PubMed
Metabolism of (R)-(+)-pulegone and (R)-(+)-menthofuran by human liver cytochrome P-450s: evidence for formation of a furan epoxide.
TLDR
Two P-450s were found to oxidize menthofuran to a newly identified metabolite, 2-hydroxymenthofuran, which is an intermediate in the formation of the known metabolites mintlactone and isomintlact one.
View on PubMed
Baculovirus expression and purification of human and rat cytochrome P450 2E1.
TLDR
High-level expression of human and rat cytochrome P450 2E1 (CYP2E1) was achieved using a baculovirus expression system and several known CYP2E2 substrates were metabolized: chlorzoxazone, p-nitrophenol, acetaminophen, and carbon tetrachloride.
View on PubMed
Electrospray ionization mass spectrometric analysis of intact cytochrome P450: identification of tienilic acid adducts to P450 2C9.
TLDR
Application of this HPLC/ESI-MS technique made the detection of the covalent modification to P450 2C9 following mechanism-based inactivation by tienilic acid possible.
View on PubMed
...
...
By clicking accept or continuing to use the site, you agree to the terms outlined in our Privacy Policy, Terms of Service, and Dataset License