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Swinging arms and swinging domains in multifunctional enzymes: catalytic machines for multistep reactions.
- R. Perham
- Chemistry, BiologyAnnual review of biochemistry
Structural and mechanistic imperatives are becoming apparent as the assembly pathways and the coupling of multistep reactions catalyzed by these dauntingly complex molecular machines are unraveled.
Redesign of the coenzyme specificity of a dehydrogenase by protein engineering
Directed mutagenesis and molecular modelling have been used to identify a set of amino-acid side chains in glutathione reductase that confer specificity for the coenzyme NADP+. Systematic replacement…
Domains, motifs, and linkers in 2-oxo acid dehydrogenase multienzyme complexes: a paradigm in the design of a multifunctional protein.
- R. Perham
- 3 September 1991
Principles of quasi-equivalence and Euclidean geometry govern the assembly of cubic and dodecahedral cores of pyruvate dehydrogenase complexes.
- T. Izard, A. Aevarsson, W. Hol
- ChemistryProceedings of the National Academy of Sciences…
- 16 February 1999
Comparison of cubic and dodecahedral E2p assemblies shows that combining the principles of quasi-equivalence formulated by Caspar and Klug with strict Euclidean geometric considerations results in predictions of the major features of the E 2p dodecahedron matching the observed features almost exactly.
A common structural motif in thiamin pyrophosphate‐binding enzymes
Protein-protein interactions in the pyruvate dehydrogenase multienzyme complex: dihydrolipoamide dehydrogenase complexed with the binding domain of dihydrolipoamide acetyltransferase.
Crystal structure of the E1 component of the Escherichia coli 2-oxoglutarate dehydrogenase multienzyme complex.
Pulling geometry defines the mechanical resistance of a beta-sheet protein.
The results reveal that the unfolding pathway depends on the pulling geometry and is associated with unfolding forces that differ by an order of magnitude, and the mechanical resistance of a protein is not dictated solely by amino acid sequence, topology or unfolding rate constant, but depends critically on the direction of the applied extension.
Identification, molecular cloning and sequence analysis of a gene cluster encoding the Class II fructose 1,6‐bisphosphate aldolase, 3‐phosphoglycerate kinase and a putative second glyceraldehyde…
To investigate a possible chromosomal clustering of glycolytic enzyme genes, the complete nucleotide sequence of the 8029 bp insert of Escherichia coli DNA in the ColE1 plasmid pLC33‐5 of the Clarke…
2‐Oxo Acid Dehydrogenase Multienzyme Complexes: Domains, Dynamics, and Design a
The E2 chains are of particular interest in that they provide the central structural and mechanistic cores of the enzyme complexes that catalyze the oxidative decarboxylation of 2-0x0 acids, releasing COz and generating the relevant acylCoA.