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Biological Relevance of a Stable Biochemical Interaction between the Tombusvirus-Encoded P19 and Short Interfering RNAs
It is demonstrated that P19/43 still suppresses RNAi-mediated viral RNA degradation in infected Nicotiana benthamiana, while P 19/75-78 is unable to prevent this clearance of viral RNA, leading to an irreversible recovery phenotype.
RNAi-associated ssRNA-specific ribonucleases in Tombusvirus P19 mutant-infected plants and evidence for a discrete siRNA-containing effector complex
This study provides biochemical evidence for a virus–host system that infection in the absence of a fully active RNAi suppressor induces ssRNA-specific ribonuclease activity, including that conferred by a RNA-induced silencing complex, which is likely the cause for the recovery of plants from infection.
The Capsid Protein of Satellite Panicum Mosaic Virus Contributes to Systemic Invasion and Interacts with Its Helper Virus
It is shown that nucleotide deletions and insertions in the 5′-UTR plus simultaneous truncation of the N-terminal part of the CP impaired SPMV spread in foxtail millet, but not in proso millet plants.
Effects of salinity on flowering, morphology, biomass accumulation and leaf metabolites in an edible halophyte
The results indicate that genotypic characteristics should be taken into account when evaluating wild plant species for future crop cultivation and highlight variations existing among Crithmum maritimum genotypes from different geographic origins regarding salt-induced changes in plant growth, flowering behavior and leaf metabolites with nutritional value.
Regulation of aldehyde oxidase and nitrate reductase in roots of barley (Hordeum vulgare L.) by nitrogen source and salinity
The barley (Hordeum vulgare) mutant Az34 which exhibited a low basal level of ABA also had a reduced capacity for abscisic acid production, and the activity of AO in roots was enhanced in Wilting mutants of tomato, plants grown with ammonium while nitrate-grown sitiens and flacca, which do not produce ABA have been plants exhibited only traces.
Aldehyde oxidase isoforms and subunit composition in roots of barley as affected by ammonium and nitrate.
Differential expression of AO subunits (160 and 145 kDa) and subsequent formation of isoforms, which differ in substrate specificity, distribution and fulfil different enzymatic reactions, may constitute an important regulatory mechanism in the plant.
Diverse and Newly Recognized Effects Associated with Short Interfering RNA Binding Site Modifications on the Tomato Bushy Stunt Virus P19 Silencing Suppressor
Even though siRNA binding by P19 is a key functional property, compromised siRNA sequestration can result in novel and diverse host-dependent properties.