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A versatile system for the expression of nonmodified bacteriocins in Escherichia coli
The aim is to develop a method and plasmid vectors suitable for expression of class II bacteriocins from Escherichia coli that are compatible with conventional vectors. Expand
Concentration of dihydrotestosterone and 3 alpha-androstanediol in naturally occurring and androgen-induced prostatic hyperplasia in the dog.
Accumulation of dihydrotestosterone is the cause of canine prostatic hyperplasia, and exogenous 3alpha-androstanediol is a better precursor of prostatic dihydotestosterone than exogenous dihydROtestosterone itself. Expand
Clinical, endocrinological, and enzymatic characterization of two patients with 5 alpha-reductase deficiency: evidence that a single enzyme is responsible for the 5 alpha-reduction of cortisol and
Two siblings with 46,XY male pseudohermapthroditism were demonstrated to have the phenotype characteristic of 5 alpha-reductase deficiency, namely normal testes and male Wolffian duct derivatives terminating in a blind-ending vagina, and studies of control and mutant epididymal microsomes indicated that a single enzyme is responsible in the normal person for the 5alpha-reduction of testosterone and cortisol. Expand
Comparison of bovine RNA polymerase III promoters for short hairpin RNA expression.
This work characterized two bovine U6 small nuclear RNA (snRNA) promoters and compared the efficiency of each of these promoters to express shRNA molecules and showed that the b7SK promoter induced the greatest level of suppression in a range of cell lines. Expand
Improved vectors for expression library immunization--application to Mycoplasma hyopneumoniae infection in pigs.
The first example of the application of ELI to a large animal model with the immunization of pigs against enzootic pneumonia, a disease caused by Mycoplasma hyopneumoniae is described. Expand
Partial characterization of the cytosol 3 alpha-hydroxysteroid: NAD(P)+oxidoreductase of rat ventral prostate.
The cytosol 3alpha-hydroxysteroid dehydrogenase of rat ventral prostate has been partially purified and conclusions have been drawn that many delta4,3-keto steroids that cannot act as substrates for the enzyme inhibit the enzyme competitively and may well serve as physiological regulators of the reaction in intact cell. Expand
Diminished 5alpha-reductase activity in extracts of fibroblasts cultured from patients with familial incomplete male pseudohermaphroditism, type 2.
The activity of 5alpha-reductase, the enzyme that converts testosterone to dihydrotestosterone, has been assessed in cell-free extracts of fibroblasts grown from foreskin, labia majora, scrotum, andExpand
Steroid 5alpha-reductase in cultured human fibroblasts. Biochemical and genetic evidence for two distinct enzyme activities.
  • R. Moore, J. Wilson
  • Medicine, Biology
  • The Journal of biological chemistry
  • 10 October 1976
Various properties of the steroid 5alpha-reductase have been examined in cell-free extracts of skin and of fibroblasts cultured from genital and nongenital skin from control subjects and fromExpand
Enhanced body growth in uremic rats treated with IGF-I and growth hormone in combination.
Investigation in female Sprague-Dawley rats with 5/6 nephrectomies found IGF-I caused significantly enhanced growth while reducing serum insulin and cholesterol levels and Creatinine clearance did not change, suggesting there were no effects of treatment on kidney function. Expand