• Publications
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Roles of ATM and NBS1 in chromatin structure modulation and DNA double-strand break repair
We developed a novel system to create DNA double-strand breaks (DSBs) at defined endogenous sites in the human genome, and used this system to detect protein recruitment and loss at and around theseExpand
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Human RECQ1 promotes restart of replication forks reversed by DNA topoisomerase I inhibition
Topoisomerase I (TOP1) inhibitors are an important class of anticancer drugs. The cytotoxicity of TOP1 inhibitors can be modulated by replication fork reversal through a process that requiresExpand
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Werner and Hutchinson–Gilford progeria syndromes: mechanistic basis of human progeroid diseases
Progeroid syndromes have been the focus of intense research in part because they might provide a window into the pathology of normal ageing. Werner syndrome and Hutchinson–Gilford progeria syndromeExpand
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Computational redesign of endonuclease DNA binding and cleavage specificity
The reprogramming of DNA-binding specificity is an important challenge for computational protein design that tests current understanding of protein–DNA recognition, and has considerable practicalExpand
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DNA binding and cleavage by the nuclear intron-encoded homing endonuclease I-PpoI
Homing endonucleases are a diverse collection of proteins that are encoded by genes with mobile, self-splicing introns. They have also been identified in self-splicing inteins (protein introns).Expand
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Genomic and Molecular Landscape of DNA Damage Repair Deficiency across The Cancer Genome Atlas
SUMMARY DNA damage repair (DDR) pathways modulate cancer risk, progression, and therapeutic response. We systematically analyzed somatic alterations to provide a comprehensive view of DDR deficiencyExpand
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Werner syndrome protein limits MYC-induced cellular senescence.
The MYC oncoprotein is a transcription factor that coordinates cell growth and division. MYC overexpression exacerbates genomic instability and sensitizes cells to apoptotic stimuli. Here weExpand
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A novel endonuclease mechanism directly visualized for I-PpoI
A novel mechanism of DNA endonucleolytic cleavage has been visualized for the homing endonuclease I-PpoI by trapping the uncleaved enzyme–substrate complex and comparing it to the previouslyExpand
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Flexible DNA target site recognition by divergent homing endonuclease isoschizomers I-CreI and I-MsoI.
Homing endonucleases are highly specific catalysts of DNA strand breaks that induce the transposition of mobile intervening sequences containing the endonuclease open reading frame. These enzymesExpand
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Design, activity, and structure of a highly specific artificial endonuclease.
We have generated an artificial highly specific endonuclease by fusing domains of homing endonucleases I-DmoI and I-CreI and creating a new 1400 A(2) protein interface between these domains. ProteinExpand
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