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Bioterrorism-related inhalational anthrax: the first 10 cases reported in the United States.
Clinical presentation and course of cases of bioterrorism-related inhalational anthrax, in the District of Columbia, Florida, New Jersey, and New York, are described; survival of patients was markedly higher than previously reported.
Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus
A real-time reverse transcription–polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome–associated coronavirus (SARS-CoV) and proved suitable to detect SARS- coV in clinical specimens.
Investigation of Bioterrorism-Related Anthrax, United States, 2001: Epidemiologic Findings
A national investigation was initiated to identify additional cases and determine possible exposures to Bacillus anthracis, and 22 cases of anthrax were identified; 5 of the inhalational cases were fatal.
Detection of pathogenic Vibrio parahaemolyticus in oyster enrichments by real time PCR.
A reassortant bunyavirus isolated from acute hemorrhagic fever cases in Kenya and Somalia.
The association of this reassortant bunyavirus, proposed name Garissa virus, with severe disease is supported by the detection of the virus RNA in acute-phase sera taken from 12 additional hemorrhagic fever cases in the region.
Detection of Bacillus anthracis DNA by LightCycler PCR
The LightCycler Bacillus anthracis assay appears to be a suitable method for rapid identification of cultured isolates of B. anthrac is directly from human specimens, and the analytical sensitivity demonstrated was 1 copy per μl of sample.
Fatal inhalational anthrax in a 94-year-old Connecticut woman.
The patient's clinical course was characterized by progression of respiratory insufficiency, pleural effusions and pulmonary edema, and, ultimately, death, and viable B anthracis was present in postmortem mediastinal lymph node specimens.
Genetic relatedness of the caliciviruses: San Miguel sea lion and vesicular exanthema of swine viruses constitute a single genotype within the Caliciviridae
Phylogenetic comparison of the Caliciviruses demonstrated that SMSV, VESV, and four related viruses are closely related while being distinct from feline calicivirus, the human calicIViruses, and rabbit hemorrhagic disease virus and that they should be classified as a single genotype within the Calingiviridae.
The capsid protein of vesicular exanthema of swine virus serotype A48: relationship to the capsid protein of other animal caliciviruses.
Evaluation and Validation of a Real-Time Polymerase Chain Reaction Assay for Rapid Identification of Bacillus anthracis
During the 2001 anthrax outbreak, a highly sensitive and specific three-target (two plasmid and one chromosomally located target) 5´ nuclease assay (real-time polymerase chain reaction [PCR]) for detection and identification of Bacillus anthracis was evaluated and validated.