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Physiological functions of mineral micronutrients (Cu, Zn, Mn, Fe, Ni, Mo, B, Cl).
This review focuses on the major functions of mineral micronutrients, mostly in cases where they were shown as constituents of proteins, making a selection and highlighting some functions in more detail. Expand
Molybdenum cofactors, enzymes and pathways
The biosynthetic pathways leading to both types of cofactor have common mechanistic aspects relating to scaffold formation, metal activation and cofactor insertion into apoenzymes, and have served as an evolutionary 'toolbox' to mediate additional cellular functions in eukaryotic metabolism. Expand
New GATEWAY vectors for high throughput analyses of protein-protein interactions by bimolecular fluorescence complementation.
- C. Gehl, Rainer Waadt, J. Kudla, R. Mendel, R. Hänsch
- Biology, Medicine
- Molecular plant
- 1 September 2009
A set of 12 GATEWAY-compatible BiFC vectors that efficiently permit the combination of candidate protein pairs with every possible N- or C-terminal sub-fragment of S(CFP)3A or Venus, respectively, and enable the performance of multicolor BiFC (mcBiFC). Expand
Identification and biochemical characterization of Arabidopsis thaliana sulfite oxidase. A new player in plant sulfur metabolism.
- T. Eilers, G. Schwarz, +7 authors R. Mendel
- Biology, Medicine
- The Journal of biological chemistry
- 14 December 2001
The cloning of a plant sulfite oxidase gene from Arabidopsis thaliana and the biochemical characterization of the encoded protein (At-SO) are reported and it is shown that a cross-reacting protein of similar size occurs in a wide range of plant species, including both herbacious and woody plants. Expand
ABA3 Is a Molybdenum Cofactor Sulfurase Required for Activation of Aldehyde Oxidase and Xanthine Dehydrogenase in Arabidopsis thaliana *
The cloning of the aba3 gene from Arabidopsis thaliana is reported and the biochemical characterization of the purified protein shows a transsulfuration reaction similar to bacterial NifS. Expand
Molybdoenzymes and molybdenum cofactor in plants.
A model for the three-step biosynthesis of Moco involving the complex interaction of six proteins will be described and a putative Moco-storage protein distributing Moco to the apoproteins of Mo-enzymes will be discussed. Expand
Sulfite Reductase Defines a Newly Discovered Bottleneck for Assimilatory Sulfate Reduction and Is Essential for Growth and Development in Arabidopsis thaliana[C][W]
The results support thatSiR is the only gene with this function in the Arabidopsis genome, that optimal activity of SiR is essential for normal growth, and that its downregulation causes severe adaptive reactions of primary and secondary metabolism. Expand
Tandem Orientation of Duplicated Xanthine Dehydrogenase Genes from Arabidopsis thaliana
- C. Hesberg, R. Hänsch, R. Mendel, F. Bittner
- Biology, Medicine
- Journal of Biological Chemistry
- 2 April 2004
It was found that AtXDH1 is a strict dehydrogenase and not an oxidase, but is able to produce superoxide radicals indicating that besides purine catabolism it might also be involved in response to various stresses that require reactive oxygen species. Expand
The Stomatal Response to Reduced Relative Humidity Requires Guard Cell-Autonomous ABA Synthesis
It is shown that guard cells possess the entire ABA biosynthesis pathway and that it appears upregulated by positive feedback by ABA, highlighting the primacy of abscisic acid (ABA) in the stomatal response to drying air. Expand
Molybdenum cofactor biosynthesis and molybdenum enzymes.
The molybdenum cofactor (Moco) forms the active site of all eukaryotic moly bdenum (Mo) enzymes and is essential for purine degradation and stress response. Expand