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A continuous spectrophotometric method for measuring the activity of serum alkaline phosphatase.
A continuous spectrophotometric method for measuring serum alkaline phosphatase activity is described. The effects of temperature, pH, substrate concentration, type and molarity of the buffer, sample… Expand
Study of optimum buffer conditions for measuring alkaline phosphatase activity in human serum.
We have compared 23 compounds, with and without transphosphorylating properties, as buffer systems for human serum alkaline phosphatase activity, with p -nitrophenylphosphate as substrate. Relative… Expand
Measurement of total alkaline phosphatase activity in human serum.
A one step Charcoal treated Alkaline phosphatase (ALP) reagent has been developed without affecting its basic ingredients. Conventionally, ALP measurement is based on the detection of phenol produced… Expand
Determination of the molar absorptivity of NADH.
- R. Mccomb, L. Bond, R. Burnett, R. C. Keech, G. Bowers
- Chemistry, Medicine
- Clinical chemistry
- 1 February 1976
The molar absorptivity of NADH at 340 nm has been determined by an indirect procedure in which high-purity glucose is phosphorylated by ATP in the presence of hexokinase, coupled to oxidation of the… Expand
Candidate reference method for determination of total bilirubin in serum: development and validation.
- B. Doumas, P. P. Kwok-Cheung, +4 authors L. Hause
- Chemistry, Medicine
- Clinical chemistry
- 1 November 1985
This candidate Reference Method for measuring total bilirubin in serum is based on the Jendrassik-Gróf principle (Clin Chem 29: 297-301, 1983). Standard Reference Material no. 916 bilirubin (National… Expand
Optimum reaction conditions for human lactate dehydrogenase isoenzymes as they affect total lactate dehydrogenase activity.
Optimum reaction conditions at 30° ± 0.5° for two continuous spectrophotometric assay procedures, lactate to pyruvate (L → P) and pyruvate to lactate (P → L), were determined with respect to pH at… Expand
Colorimetric estimation of d-glucose and 2-deoxy-d-glucose with glucose oxidase
- R. Mccomb, W. D. Yushok
- Mathematics
- 1 May 1958
Abstract A modification of Keston's glucose oxidase method for d -glucose analysis is described. The glucose oxidase-peroxidase reaction was carried out in phosphate buffer, pH 5.9 at 45°, for 30… Expand
High-purity 4-nitrophenol: purification, characterization, and specifications for use as a spectrophotometric reference material.
- G. Bowers, R. Mccomb, R. G. Christensen, R. Schaffer
- Chemistry, Medicine
- Clinical chemistry
- 1 May 1980
We describe specifications for high-purity 4-nitrophenol, which is suitable for spectrophotometric standardization. Such a reference material is needed in clinical enzymology to establish the proper… Expand
METABOLISM OF ASCITES TUMOR CELLS. III. EFFECT OF 2-DEOXYGLUCOSE PHOSPHORYLATION ON PHOSPHORUS METABOLISM.
- R. Mccomb, W. D. Yushok
- Biology, Medicine
- Cancer research
- 1 February 1964