Francisella tularensis LVS evades killing by human neutrophils via inhibition of the respiratory burst and phagosome escape
The data are the first demonstration of a facultative intracellular pathogen, which disrupts the oxidative burst and escapes the phagosome to evade elimination inside neutrophils, and as such, define a novel mechanism of virulence.
Identification of migR, a Regulatory Element of the Francisella tularensis Live Vaccine Strain iglABCD Virulence Operon Required for Normal Replication and Trafficking in Macrophages
MigR, a gene that regulates expression of the iglABCD operon and is essential for bacterial growth in MDMs and also contributes to the blockade of neutrophil NADPH oxidase activity is identified.
Multiple mechanisms of NADPH oxidase inhibition by type A and type B Francisella tularensis
Several type A and type B Ft strains are used to demonstrate that Ft‐mediated NADPH oxidase inhibition is more complex than appreciated previously, and data strongly suggest that this is a central aspect of virulence.
Francisella tularensis Genes Required for Inhibition of the Neutrophil Respiratory Burst and Intramacrophage Growth Identified by Random Transposon Mutagenesis of Strain LVS
It is shown that uracil auxotrophy has cell type-specific effects on the fate of Francisella bacteria, and random transposon mutagenesis is used to identify LVS genes that affect neutrophil activation.
A specific gene expression program triggered by Gram-positive bacteria in the cytosol.
- R. McCaffrey, P. Fawcett, D. Portnoy
- BiologyProceedings of the National Academy of Sciences…
- 3 August 2004
This study identified a cytosol-specific transcriptional program independent of TLR signaling through MyD88, and its points of convergence with TLR- and IFN-dependent pathways will enhance the understanding of the means by which mammals detect and respond to pathogens.
The host response to adenovirus, helper-dependent adenovirus, and adeno-associated virus in mouse liver.
The results indicate that recognition of the Ad capsid or double-stranded DNA in the vector elicits a robust type I IFN response that is, however, not elicited by AAV-derived vector transduction.
The role of complement opsonization in interactions between F. tularensis subsp. novicida and human neutrophils.
To activate or not to activate: distinct strategies used by Helicobacter pylori and Francisella tularensis to modulate the NADPH oxidase and survive in human neutrophils
The novel strategies used by Helicobacter pylori and Francisella tularensis to disrupt neutrophil function are described, with a focus on assembly and activation of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase.
Francisella tularensis Modulates a Distinct Subset of Regulatory Factors and Sustains Mitochondrial Integrity to Impair Human Neutrophil Apoptosis
- J. McCracken, Lauren C. Kinkead, R. McCaffrey, L. Allen
- Biology, MedicineJournal of Innate Immunity
- 24 February 2016
unique features of this infection are identified and fundamental insight into the mechanisms of apoptosis inhibition is provided, which advance the understanding of neutrophil apoptosis and its capacity to be manipulated by pathogenic bacteria.
Incidence, Etiology and Risk Factors for Travelers’ Diarrhea during a Hospital Ship-Based Military Humanitarian Mission: Continuing Promise 2011
Despite employment of current and targeted preventive interventions, ship-board HA/DR missions may experience a significant risk for TD among deployed US military personnel and potentially impact mission success.