Stable fluorescent complexes of double-stranded DNA with bis-intercalating asymmetric cyanine dyes: properties and applications.
The synthesis, proof of structure, and the absorption and fluorescence properties of two new unsymmetrical cyanine dyes, thiazole orange dimer and oxazole yellow dimer are reported, which form highly fluorescent complexes with double-stranded DNA (dsDNA) with greater than 1000-fold fluorescence enhancement.
Monolithic membrane valves and diaphragm pumps for practical large-scale integration into glass microfluidic devices
Conical intersection dynamics of the primary photoisomerization event in vision
Ultrafast optical spectroscopy with sub-20-fs time resolution and spectral coverage from the visible to the near-infrared allows us to follow the dynamics leading to the conical intersection in rhodopsin isomerization and finds excellent agreement between the experimental observations and molecular dynamics calculations that involve a true electronic state crossing.
The first step in vision: femtosecond isomerization of rhodopsin.
Measurements demonstrate that the first step in vision, the 11-cis----11-trans torsional isomerization of the rhodopsin chromophore, is essentially complete in only 200 femtoseconds.
Femtosecond Stimulated Raman Spectroscopy.
The historic overview and discusses the theoretical and experimental concepts behind FSRS, and emphasis is put on the current state-of-the-art experimental realization and several variations of FSRS that have been developed.
Retinal counterion switch in the photoactivation of the G protein-coupled receptor rhodopsin
- Elsa C. Y. Yan, M. Kazmi, R. Mathies
- Chemistry, BiologyProceedings of the National Academy of Sciences…
- 30 June 2003
A model where the counterion switch occurs by transferring a proton from GLU-181 to Glu-113 through an H-bond network formed primarily with residues on extracellular loop II (EII) is proposed, which may be a general element of G protein-coupled receptor activation.
Surface multiheme c-type cytochromes from Thermincola potens and implications for respiratory metal reduction by Gram-positive bacteria
- H. Carlson, A. Iavarone, J. Coates
- BiologyProceedings of the National Academy of Sciences
- 17 January 2012
These results provide unique direct evidence for cell wall-associated cytochromes and support MHC involvement in conducting electrons across the cell envelope of a Gram-positive bacterium.
From femtoseconds to biology: mechanism of bacteriorhodopsin's light-driven proton pump.
- R. Mathies, S. W. Lin, J. Ames, W. T. Pollard
- ChemistryAnnual Review of Biophysics and Biophysical…
Resonance Raman vibrational spectroscopy has been used to determine the structure of the chromophore in each of bacteriorhodopsin’s intermediates and to analyze the kinetics of the photocycle, and these results are integrated into an explicit molecular model for proton pumping in bacterior Hodopsin.
Direct observation of the femtosecond excited-state cis-trans isomerization in bacteriorhodopsin.
Femtosecond optical measurement techniques have been used to study the primary photoprocesses in the light-driven transmembrane proton pump bacteriorhodopsin. Light-adapted bacteriorhodopsin was…
Femtosecond broadband stimulated Raman spectroscopy: Apparatus and methods.
- D. McCamant, P. Kukura, Sangwoon Yoon, R. Mathies
- Chemistry, PhysicsThe Review of scientific instruments
- 2 November 2004
The dependence of the stimulated Raman signal on experimental parameters is explored, demonstrating the expected exponential increase in Raman intensity with concentration, pathlength, and Raman pump power.