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Multiplex Real-Time PCR for Detection of Anaplasma phagocytophilum and Borrelia burgdorferi
ABSTRACT A multiplex real-time PCR assay was developed for the simultaneous detection of Anaplasma phagocytophilum and Borrelia burgdorferi. The assay was tested on various Anaplasma, Borrelia,Expand
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Diagnosis and management of Q fever--United States, 2013: recommendations from CDC and the Q Fever Working Group.
Q fever, a zoonotic disease caused by the bacterium Coxiella burnetii, can cause acute or chronic illness in humans. Transmission occurs primarily through inhalation of aerosols from contaminatedExpand
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Nested PCR Assay for Detection of Granulocytic Ehrlichiae
ABSTRACT A sensitive and specific nested PCR assay was developed for the detection of granulocytic ehrlichiae. The assay amplifies the 16S rRNA gene and was used to examine acute-phase EDTA-blood andExpand
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Etiology of Severe Non-malaria Febrile Illness in Northern Tanzania: A Prospective Cohort Study
Introduction The syndrome of fever is a commonly presenting complaint among persons seeking healthcare in low-resource areas, yet the public health community has not approached fever in aExpand
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PCR Detection of Granulocytic Ehrlichiae in Ixodes ricinus Ticks and Wild Small Mammals in Western Switzerland
ABSTRACT The presence of granulocytic ehrlichiae was demonstrated by PCR inIxodes ricinus ticks and wild small mammals in Switzerland in two areas of endemicity for bovine ehrlichiosis. Six ticksExpand
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Diagnosis and Management of Tickborne Rickettsial Diseases: Rocky Mountain Spotted Fever and Other Spotted Fever Group Rickettsioses, Ehrlichioses, and Anaplasmosis - United States.
Tickborne rickettsial diseases continue to cause severe illness and death in otherwise healthy adults and children, despite the availability of low-cost, effective antibacterial therapy. RecognitionExpand
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Rocky mountain spotted fever in the United States, 2000-2007: interpreting contemporary increases in incidence.
Rocky Mountain spotted fever (RMSF), a potentially fatal tick-borne infection caused by Rickettsia rickettsii, is considered a notifiable condition in the United States. During 2000 to 2007, theExpand
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Sequence Analysis of the msp4 Gene of Anaplasma phagocytophilum Strains
ABSTRACT The causative agent of human granulocytic ehrlichiosis was recently reclassified as Anaplasma phagocytophilum, unifying previously described bacteria that cause disease in humans, horses,Expand
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Analysis of the complete genome of smallpox variola major virus strain Bangladesh-1975.
We analyzed the 186,102 base pairs (bp) that constitute the entire DNA genome of a highly virulent variola virus isolated from Bangladesh in 1975. The linear, double-stranded molecule has relativelyExpand
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Seroepidemiologic and occupational risk survey for Coxiella burnetii antibodies among US veterinarians.
BACKGROUND Little is known about the occurrence of Q fever among veterinarians in the United States. In this study, we sought to estimate the prevalence of Coxiella burnetii antibodies amongExpand
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