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Kinetic studies on the interaction of eglin c with human leukocyte elastase and cathepsin G.
TLDR
From the above kinetic constants, it may be inferred that at a therapeutic concentration of 5 X 10(-7) M, Eglin c will inhibit leukocyte elastase in one second and will bind this enzyme in a "pseudo-irreversible" manner.
The complete covalent structure of hirudin. Localization of the disulfide bonds.
TLDR
Hirudin seems to be unique in its primary structure and hence designates an unknown inhibitor family, due to the lack of any closer sequence homology and topological structural homology to other serine proteinase inhibitor proteins.
Primary structure and function of novel O-glycosylated hirudins from the leech Hirudinaria manillensis.
TLDR
The primary structure of two hirudins from the leech Hirudinaria manillensis has been elucidated and shows an interesting similarity to human blood group H determinants.
Eglin c, a pharmacologically active elastase inhibitor.
TLDR
The rate of complex formation between Eglin c and human leukocyte elastase (HLE) or human cathepsin G (H. Cat. G) was determined and compared to those of a number of other proteinase/proteinase-inhibitor interactions (alpha 1 PI and alpha 2M).
Eglin-c, a polypeptide derived from the medicinal leech, prevents human neutrophil elastase-induced emphysema and bronchial secretory cell metaplasia in the hamster.
TLDR
Eglin-c (Eg-c), a polypeptide with a molecular mass of 8,100 daltons, was purified from the medicinal leech Hirudo medicinalis by reductive methylation for in vitro studies and formed a 34,000-dalton complex that was observed in in vivo studies of golden Syrian hamsters.
Function of individual 30S subunit proteins of Escherichia coli. Effect of specific immunoglobulin fragments (Fab) on activities of ribosomal decoding sites.
Specific anti-30S protein immunoglobulin G fragments (Fab) were used to determine the contribution of each of the 30S ribosomal proteins to: (1) polyphenylalanine synthesis, (2) initiation
Isolation and characterization of hirudin isoinhibitors and sequence analysis of hirudin PA.
TLDR
The final purification of two thrombin-inhibiting preparations by reversed-phase high-performance liquid chromatography yielded several isohirudins with either N-terminal valine or isoleucine but with identical inhibition characteristics, i.e. specific throm bin inhibiting activities of 680-720 IU/mg.
Inhibition of cells in culture by polyamines does not depend on the presence of ruminant serum.
TLDR
In vitro inhibition by polyamines was shown to require neither foetal calf serum (FCS) nor the addition of any exogenous polyamine oxidase, and evidence is presented to show that the source of the enzyme is the cells themselves.
Screening for granulopoiesis inhibitors (chalones) by different assays.
TLDR
Comparison of the test systems revealed that the granulocyte colony assay may detect inhibitors different from those found by the [3H]thymidine bone-marrow assay; the validity of the latter test is seriously questioned, however.
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