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Expression of a family of noncoding mitochondrial RNAs distinguishes normal from cancer cells
We reported the presence in human cells of a noncoding mitochondrial RNA that contains an inverted repeat (IR) of 815 nucleotides (nt) covalently linked to the 5′ end of the mitochondrial 16S RNAExpand
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Nuclear localization of the mitochondrial ncRNAs in normal and cancer cells
BackgroundWe have previously shown a differential expression of a family of mitochondrial ncRNAs in normal and cancer cells. Normal proliferating cells and cancer cells express the senseExpand
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An efficient transformation method for Bacillus subtilis DB104
Bacillus subtilis strains are used for extracellular expression of enzymes (i.e., proteases, lipases, and cellulases) which are often engineered by directed evolution for industrial applications. B.Expand
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Surface charge engineering of a Bacillus gibsonii subtilisin protease
In proteins, a posttranslational deamidation process converts asparagine (Asn) and glutamine (Gln) residues into negatively charged aspartic (Asp) and glutamic acid (Glu), respectively. This processExpand
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Expression of a novel non-coding mitochondrial RNA in human proliferating cells
Previously, we reported the presence in mouse cells of a mitochondrial RNA which contains an inverted repeat (IR) of 121 nucleotides (nt) covalently linked to the 5′ end of the mitochondrial 16S RNAExpand
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A first continuous 4-aminoantipyrine (4-AAP)-based screening system for directed esterase evolution
Esterases hydrolyze ester bonds with an often high stereoselectivity as well as regioselectivity and are therefore industrially employed in the synthesis of pharmaceuticals, in food processing, andExpand
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Enzyme-assisted extraction of proteins from the seaweeds Macrocystis pyrifera and Chondracanthus chamissoi: characterization of the extracts and their bioactive potential
Seaweeds have great potential as raw material for protein-based functional ingredients due to their high protein content; however, their complex polysaccharide matrix could hinder protein extraction.Expand
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