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A Flow Cytometry–Based Screening System for Directed Evolution of Proteases
Proteases are industrially important enzymes but often have to be improved for their catalytic efficiency and stabilities to suit applications. Flow cytometry screening technology based on in vitroExpand
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Increasing activity and thermal resistance of Bacillus gibsonii alkaline protease (BgAP) by directed evolution
Bacillus gibsonii Alkaline Protease (BgAP) is a recently reported subtilisin protease exhibiting activity and stability properties suitable for applications in laundry and dish washing detergents.Expand
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A roadmap to directed enzyme evolution and screening systems for biotechnological applications.
TLDR
We present and discuss the advantages and challenges of designing and performing a directed enzyme evolution campaign, current advances in methods, as well as highlighting some examples of its applications in industrially relevant enzymes. Expand
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Inhibition of iron and copper uptake by iron, copper and zinc.
Interactions of micronutrients can affect absorption and bioavailability of other nutrients by a number of mechanisms. In aqueous solutions, and at higher uptake levels, competition between elementsExpand
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Ionic liquid and deep eutectic solvent-activated CelA2 variants generated by directed evolution
Chemoenzymatic cellulose degradation is one of the key steps for the production of biomass-based fuels under mild conditions. An effective cellulose degradation process requires diverseExpand
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Directed evolution of P450cin for mediated electron transfer
Directed evolution is a powerful method to optimize enzyme properties for application demands. Interesting targets are P450 monooxygenases which catalyze the stereo- and regiospecific hydroxylationExpand
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A flow cytometer-based whole cell screening toolbox for directed hydrolase evolution through fluorescent hydrogels.
A high throughput whole cell flow cytometer screening toolbox was developed and validated by identifying improved variants (1.3-7-fold) for three hydrolases (esterase, lipase, cellulase). TheExpand
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Correction: A flow cytometer-based whole cell screening toolbox for directed hydrolase evolution through fluorescent hydrogels.
Correction for 'A flow cytometer-based whole cell screening toolbox for directed hydrolase evolution through fluorescent hydrogels' by Nina Lülsdorf et al., Chem. Commun., 2015, 51, 8679-8682.
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Insights on activity and stability of subtilisin E towards guanidinium chloride and sodium dodecylsulfate.
A subtilisin E variant (M4) showing high activity and resistance towards guanidinium chloride (GdmCl) and sodium dodecylsulfate (SDS) was previously identified after three rounds of directedExpand
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Redirecting catalysis from proteolysis to perhydrolysis in subtilisin Carlsberg.
Enzyme promiscuity describes the ability of biocatalysts to catalyze conversions beyond their natural reactions. Enzyme engineering to promote side reactions is attractive for synthetic andExpand
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