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A Third-Generation Lentivirus Vector with a Conditional Packaging System
TLDR
It is demonstrated that the requirement for the tat gene can be offset by placing constitutive promoters upstream of the vector transcript, and the improved design presented here should facilitate testing of lentivirus vectors.
Self-Inactivating Lentivirus Vector for Safe and Efficient In Vivo Gene Delivery
TLDR
The inactivation design achieved in this work improves significantly the biosafety of HIV-derived vectors, as it reduces the likelihood that replication-competent retroviruses will originate in the vector producer and target cells, and hampers recombination with wild-type HIV in an infected host.
Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo
TLDR
An HIV vector system in which the virulence genes env, vif, vpr, vpu, and nef have been deleted is described, and this multiply attenuated vector conserved the ability to transduce growth-arrested cells and monocyte-derived macrophages in culture, and could efficiently deliver genes in vivo into adult neurons.
Nucleus basalis and thalamic control of neocortical activity in the freely moving rat
TLDR
It is suggested that the NB system may serve as a structural basis for the concept of the generalized ascending activation of Moruzzi and Magoun (1949) by directly activating the neocortex and by suppressing the rhythm generation in the RT-thalamocortical circuitry.
Parkinson-Like Neurodegeneration Induced by Targeted Overexpression of α-Synuclein in the Nigrostriatal System
TLDR
It is shown that nigral dopamine neurons are selectively vulnerable to high levels of either wild-type or mutant α-synuclein, pointing to a key role for α- Synuclein in the pathogenesis of Parkinson's disease.
Long-Term rAAV-Mediated Gene Transfer of GDNF in the Rat Parkinson's Model: Intrastriatal But Not Intranigral Transduction Promotes Functional Regeneration in the Lesioned Nigrostriatal System
TLDR
The results demonstrate that both nigral and striatal transduction provide significant protection of nigral DA neurons against the toxin-induced degeneration, and provide evidence that rAAV is a highly efficient vector system for long-term expression of therapeutic proteins in the nigrostriatal system.
The phosphorylation state of Ser-129 in human α-synuclein determines neurodegeneration in a rat model of Parkinson disease
TLDR
The nonphosphorylated form of S129 exacerbates α-syn-induced nigral pathology, whereas Ser-129 phosphorylation eliminates α- synchronized nigrostriatal degeneration, which suggests possible new therapeutic targets for Parkinson Disease.
Continuous Low-Level Glial Cell Line-Derived Neurotrophic Factor Delivery Using Recombinant Adeno-Associated Viral Vectors Provides Neuroprotection and Induces Behavioral Recovery in a Primate Model
TLDR
It is concluded that when delivered continuously, a low level of GDNF in the striatum (approximately threefold above baseline) is sufficient to provide optimal functional outcome.
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