How bacteria assemble flagella.
- R. Macnab
- BiologyAnnual Review of Microbiology
- 28 November 2003
The bacterial flagellum is both a motor organelle and a protein export/assembly apparatus that extends from the cytoplasm to the cell exterior and employs a type III pathway, utilized for secretion of virulence factors.
Genetics and biogenesis of bacterial flagella.
- R. Macnab
- BiologyAnnual Review of Genetics
- 1992
pH homeostasis in Escherichia coli: measurement by 31P nuclear magnetic resonance of methylphosphonate and phosphate.
- J. Slonczewski, B. Rosen, J. Alger, R. Macnab
- BiologyProceedings of the National Academy of Sciences…
- 1 October 1981
The pH-sensitive NMR probe methylphosphonate was employed, which was taken up by glycerol-grown cells and was nontoxic; its pKa of 7.65 made it an ideal probe for measurement of cytoplasmic pH and alkaline external pH.
Domain Structure of Salmonella FlhB, a Flagellar Export Component Responsible for Substrate Specificity Switching
- T. Minamino, R. Macnab
- BiologyJournal of Bacteriology
- 1 September 2000
It is proposed that FlhB(C), a component of the type III flagellar export apparatus, has two substrate specificity states and that a conformational change, mediated by the interaction between Fl hB(CN) and Flhb(CC), is responsible for the specificity switching process.
The gradient-sensing mechanism in bacterial chemotaxis.
- R. Macnab, D. Koshland
- PhysicsProceedings of the National Academy of Sciences…
- 1 September 1972
It was found, however, that a sudden increase also elicits a response, namely supercoordinated swimming, which demonstrates that chemotaxis is achieved by modulation of the incidence of tumbling both above and below its steady-state value.
Mutations in fliK and flhB affecting flagellar hook and filament assembly in Salmonella typhimurium
- A. Williams, S. Yamaguchi, F. Togashi, S. Aizawa, I. Kawagishi, R. Macnab
- BiologyJournal of Bacteriology
- 1 May 1996
It is suggested that FliK measures hook length and, at the appropriate point, sends a signal to FlhB to switch the substrate specificity of export from hook protein to late proteins such as flagellin.
Components of the Salmonella Flagellar Export Apparatus and Classification of Export Substrates
- T. Minamino, R. Macnab
- BiologyJournal of Bacteriology
- 1 March 1999
It was established that there is an interaction between the processes of outer ring assembly and of penetration of the outer membrane by the rod and nascent hook, the latter process being of course necessary for passage of export substrates into the external medium.
Localization of the Salmonella typhimurium flagellar switch protein FliG to the cytoplasmic M-ring face of the basal body.
- N. Francis, V. M. Irikura, S. Yamaguchi, D. DeRosier, R. Macnab
- BiologyProceedings of the National Academy of Sciences…
- 15 July 1992
FliG, one of the switch proteins of Salmonella typhimurium, is localized to the cytoplasmic face of the M ring of the flagellar basal body and the stoichiometric ratio between FliF and FliG in wild-type cells is probably 1:1.
Interactions among membrane and soluble components of the flagellar export apparatus of Salmonella.
- Keng Zhu, B. González-Pedrajo, R. Macnab
- BiologyBiochemistry
- 4 July 2002
Interactions among several components of the flagellar export apparatus of Salmonella were studied using affinity chromatography, affinity blotting, and fluorescence resonance energy transfer (FRET) to reveal the nature of the binding interactions.
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