• Publications
  • Influence
Effects of lovastatin (mevinolin) on sterol levels and on activity of azoles in Saccharomyces cerevisiae.
  • R. Lorenz, L. Parks
  • Biology, Medicine
  • Antimicrobial Agents and Chemotherapy
  • 1 September 1990
The hypocholesterolemic drug lovastatin (mevinolin) was found to be very effective in lowering the sterol levels of the wild-type yeast Saccharomyces cerevisiae. Lovastatin dramatically decreased theExpand
  • 86
  • 6
Cloning, sequencing, and disruption of the gene encoding sterol C-14 reductase in Saccharomyces cerevisiae.
A sterol C-14 reductase (erg24-1) mutant of Saccharomyces cerevisiae was selected in a fen1, fen2, suppressor background on the basis of nystatin resistance and ignosterol (ergosta-8,14-dienol)Expand
  • 60
  • 3
Physiological effects of fenpropimorph on wild-type Saccharomyces cerevisiae and fenpropimorph-resistant mutants.
  • R. Lorenz, L. Parks
  • Biology, Medicine
  • Antimicrobial Agents and Chemotherapy
  • 1 August 1991
Fenpropimorph-resistant mutants of Saccharomyces cerevisiae were isolated by a gradient selection procedure. The mutants were cross-resistant to other morpholines (fenpropidin, dodemorph, tridemorph)Expand
  • 36
  • 2
Characteristics of sterol uptake in Saccharomyces cerevisiae.
A Saccharomyces cerevisiae sterol auxotroph, FY3 (alpha hem1 erg7 ura), was used to probe the characteristics of sterol uptake in S. cerevisiae. The steady-state cellular concentration of free sterolExpand
  • 48
Structural discrimination in the sparking function of sterols in the yeast Saccharomyces cerevisiae.
A Saccharomyces cerevisiae sterol auxotroph, SPK14 (a hem1 erg6 erg7 ura), was constructed to test the ability of selected C-5,6 unsaturated sterols at growth-limiting concentrations to spark growthExpand
  • 43
Involvement of heme components in sterol metabolism ofSaccharomyces cerevisiae
There is an intimate association between sterol biosynthesis in yeast and aerobicity. Besides the requirement for molecular oxygen for the epoxidation of squalene, cytochrome hemoproteins areExpand
  • 46
Regulation of ergosterol biosynthesis and sterol uptake in a sterol-auxotrophic yeast.
Inhibition of sterol uptake in Saccharomyces cerevisiae sterol auxotroph FY3 (alpha hem1 erg7 ura) by delta-aminolevulinic acid (ALA) is dependent on the ability of the organism to synthesize hemeExpand
  • 25
Fenpropimorph affects uptake of uracil and cytosine in Saccharomyces cerevisiae.
Fenpropimorph was shown to inhibit the accumulation of the pyrimidine bases uracil and cytosine from the growth media in Saccharomyces cerevisiae. Uracil prototrophs of S. cerevisiae were moreExpand
  • 11
Functions for Sterols in Yeast Membranes
Most of the clinically useful antifungal agents either interact directly with the sterols in the fungal membrane or interfere with the synthesis of fungal sterols. The characteristics of these agentsExpand
  • 4