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Development of a Chinese Hamster Ovary Cell Line for Recombinant Adenovirus‐Mediated Gene Expression
Recombinant human adenovirus (rhAd) has been used extensively for functional protein expression in mammalian cells including those of human and nonhuman origin. High‐level protein production by rhAd… Expand
Zinc supplementation protects human endostatin Fc fusion against proteolytic degradation during cell culture.
- G. Hao, Jessica Dawson, G. Lee, R. Longley
- Chemistry, Medicine
- Protein expression and purification
Endostatin is a potent anti-angiogenesis compound with efficacy in treating solid tumors and other diseases. However, its clinical application has been hampered by the susceptibility to proteolytic… Expand
Development of a Serum-free Suspension Process for the Production of a Conditionally Replicating Adenovirus using A549 Cells
Conditionally replicating adenoviruses (CRAVs) are a group of recombinant human adenoviruses genetically engineered to replicate in selected tissues, such as tumors. These viruses could potentially… Expand
Isometachromin, a new cytotoxic sesquiterpenoid from a deep water sponge of the family Spongiidae
Isometachromin (1), a new sesquiterpene-quinone that is related structurally to metachromin C (2), and the known compounds ilimaquinone (3) and and 5-epi-ilimaquinone (4), were isolated from a deep… Expand
View from the boardroom. Interview by Sarah F. Zarbock.
- Robert F. Longley
- Home care provider
When it comes to home care agencies and their boards of directors, I've sat in both chairs. As a physician assistant (PA), I provided home care directly when I worked for a multispecialty physician… Expand
and ANTHRONE REAGENT IN LIVER AND MUSCLE BY USE OF THE DETERMINATION OF GLYCOGEN
In currently used methods for the determination of glycogen, tissue is extracted either by boiling with 30 per cent potassium hydroxide solution (KOH) or by homogenization with trichloroacetic acid… Expand
National Commission on Libraries (1991): Correspondence 43
- Robert F. Longley
- Political Science