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Purine metabolism in Toxoplasma gondii.
We have studied the incorporation and interconversion of purines into nucleotides by freshly isolated Toxoplasma gondii. They did not synthesize nucleotides from formate, glycine, or serine. TheExpand
A simple monophasic medium for axenic culture of hemoflagellates.
An inexpensive, easily prepared monophasic medium which supports continuous culture of Leishmania donovanì (approximately 4 X 10(7) cells/ml/day 5) and L. tarentolae (approximately 3.5 X 10(7)Expand
Inhibition of growth of Toxoplasma gondii by qinghaosu and derivatives.
The antimalarial compound qinghaosu (artemisinin) was tested in vitro for the ability to inhibit plaque formation by Toxoplasma gondii in fibroblasts. Qinghaosu at 0.4 microgram/ml for 5 daysExpand
Purine metabolism in Leishmania donovani and Leishmania braziliensis.
We have studied purine metabolism in the culture forms of Leishmania donovani and Leishmania braziliensis. These organisms are incapable of synthesizing purines de novo from glycine, serine, orExpand
Antitrypanosomal effect of allopurinol: conversion in vivo to aminopyrazolopyrimidine nucleotides by Trypanosoma curzi.
The parasite Trypanosoma cruzi metabolizes allopurinol by a sequential conversion to allopurinol mononucleotide and aminopurinol mononucleotide. The latter is incorporated into RNA. ThisExpand
Purine and pyrimidine salvage pathways in Leishmania donovani.
Leishmania donovani, grown in culture, salvaged radiolabeled purine bases which were distributed into adenine and guanine ribonucleotides and into the RNA of these cells. De novo synthesis of purinesExpand
Purine metabolism in the bloodstream forms of Trypanosoma gambiense and Trypanosoma rhodesiense.
Bloodstream forms of Trypanosoma brucei gambiense and Trypanosoma brucei rhodesiense are incapable of de novo purine synthesis. Purine bases are converted directly to ribonucleotides and with theExpand
Amplification of the inosinate dehydrogenase gene in Trypanosoma brucei gambiense due to an increase in chromosome copy number.
Drug resistance is a significant impediment to the therapy of African sleeping sickness in humans. To evaluate molecular mechanisms that contribute to drug resistance in African trypanosomes, aExpand
Adenosine analog metabolism in Giardia lamblia. Implications for chemotherapy.
Certain adenosine analogs can inhibit the growth of Giardia lamblia. This biological action correlates with the ability of the organism to phosphorylate the nucleoside directly to the nucleotide.Expand
Allopurinol ribonucleoside as an antileishmanial agent. Biological effects, metabolism, and enzymatic phosphorylation.