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Trypanosomes lacking trypanothione reductase are avirulent and show increased sensitivity to oxidative stress
TLDR
The infectivity and virulence of the parasites in mice was dependent upon tetracycline‐regulated TRYR activity: if the trypanosomes were injected into mice in the absence of tetrACYcline, no infection was detectable; and when tetr Tracycline was withdrawn from previously infected animals, the parasitaemia was suppressed.
Overexpression of the putative thiol conjugate transporter TbMRPA causes melarsoprol resistance in Trypanosoma brucei
TLDR
The characterization of two genes encoding putative metal–thiol conjugate transporters from Trypanosoma brucei are described, which gave two‐ to fourfold melarsoprol resistance, but did not enhance resistance caused by MRPA.
The Dithiol Glutaredoxins of African Trypanosomes Have Distinct Roles and Are Closely Linked to the Unique Trypanothione Metabolism*
TLDR
The RNA interference against Grx2 caused a growth retardation of procyclic cells consistent with an essential role in the trypanothione-based thiol redox metabolism of African trypanosomes.
Biological Activities of Xanthatin from Xanthium strumarium Leaves
TLDR
Data taken together suggest that xanthatin exerts its biological activity by inducing apoptosis and inhibiting both PGE2 synthesis and 5‐lipoxygenase activity thereby avoiding unwanted inflammation commonly observed in diseases such as trypanosomiasis.
Trypanothione-dependent Synthesis of Deoxyribonucleotides by Trypanosoma brucei Ribonucleotide Reductase*
TLDR
It is shown that the dithiol trypanothione (bis(glutathionyl)spermidine), in contrast to glutathione, is a direct reductant of T. brucei ribonucleotide reductase with a K m value of 2 mm, the first example of a natural low molecular mass thiol directly delivering reducing equivalents for ribon nucleotide reduction.
2- and 3-substituted 1,4-naphthoquinone derivatives as subversive substrates of trypanothione reductase and lipoamide dehydrogenase from Trypanosoma cruzi: synthesis and correlation between redox
TLDR
The results obtained here confirm that reduction of NQs by parasitic flavoenzymes is a promising strategy for the development of new trypanocidal drugs.
Substrate Specificity, Localization, and Essential Role of the Glutathione Peroxidase-type Tryparedoxin Peroxidases in Trypanosoma brucei*
TLDR
Trypanosoma brucei, the causative agent of African sleeping sickness, encodes three nearly identical cysteine homologues of the classical selenocysteine-containing glutathione peroxidases, which causes severe growth defects in bloodstream and procyclic cells in accordance with the peroxIDases being essential in both developmental stages.
A Second Class of Peroxidases Linked to the Trypanothione Metabolism*
TLDR
The catalytic efficiency of theperoxidase studied here is comparable with that of the peroxiredoxin-like tryparedoxin peroxidases, which shows that trypanosomes possess two distinct peroxIDase systems both dependent on the unique dithiol trypanothione.
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