• Publications
  • Influence
Structural Basis of Transcription: RNA Polymerase II at 2.8 Ångstrom Resolution
TLDR
Structures of a 10-subunit yeast RNA polymerase II derived from two crystal forms at 2.8 and 3.1 angstrom resolution provide evidence for RNA exit in the vicinity of the carboxyl-terminal repeat domain, coupling synthesis to RNA processing by enzymes bound to this domain.
Structural Basis of Transcription: An RNA Polymerase II Elongation Complex at 3.3 Å Resolution
TLDR
The crystal structure of RNA polymerase II in the act of transcription was determined at 3.3 Å resolution and protein–nucleic acid contacts help explain DNA and RNA strand contacts, the specificity of RNA synthesis, “abortive cycling” during transcription initiation, and RNA and DNA translocation during transcription elongation.
Chromatin structure: a repeating unit of histones and DNA.
TLDR
Preliminary results do show less cross-linking of histones in chromatin than in solution, but crosslinked products up to pentamers are readily observed and call for further investigation.
Architecture of RNA polymerase II and implications for the transcription mechanism.
TLDR
A backbone model of a 10-subunit yeast RNA polymerase II has been derived from x-ray diffraction data extending to 3 angstroms resolution, and features of the model include a pair of jaws that appear to grip DNA downstream of the active center.
Structural Basis of Transcription: An RNA Polymerase II-TFIIB Cocrystal at 4.5 Angstroms
The structure of the general transcription factor IIB (TFIIB) in a complex with RNA polymerase II reveals three features crucial for transcription initiation: an N-terminal zinc ribbon domain of
...
1
2
3
4
5
...