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Recognition of Antimicrobial Peptides by a Bacterial Sensor Kinase
TLDR
It is shown that enzymatic activity of Salmonella typhimurium PhoQ is directly activated by antimicrobial peptides. Expand
Mutant adenosine deaminase 2 in a polyarteritis nodosa vasculopathy.
TLDR
Recessive loss-of-function mutations of ADA2, a growth factor that is the major extracellular adenosine deaminase, can cause polyarteritis nodosa vasculopathy with highly varied clinical expression. Expand
UbcH7 reactivity profile reveals Parkin and HHARI to be RING/HECT hybrids
TLDR
It is shown that, unlike many E2s that transfer Ub with RINGs, UBCH7 lacks intrinsic, E3-independent reactivity with lysine, explaining its preference for HECTs, and RBRs function like RING/HECT hybrids, indicating a novel mechanism for an entire class of E3s. Expand
Binding and recognition in the assembly of an active BRCA1/BARD1 ubiquitin-ligase complex
TLDR
Using NMR spectroscopy and site-directed mutagenesis, the binding site on the BRCA1/BARD1 heterodimer for the Ub-conjugating enzyme UbcH5c is mapped and it is demonstrated that binding alone is not sufficient for BRCa1-dependent Ub-ligase activity. Expand
Structure of a BRCA1–BARD1 heterodimeric RING–RING complex
TLDR
The BRCA1–BARD1 structure provides a model for its ubiquitin ligase activity, illustrates how the BRCa1 RING domain can be involved in associations with multiple protein partners and provides a framework for understanding cancer-causing mutations at the molecular level. Expand
RING-type E3 ligases: master manipulators of E2 ubiquitin-conjugating enzymes and ubiquitination.
TLDR
Recent advances in RING-type E3 recognition of substrates, their cellular regulation, and their varied architecture are reviewed. Expand
E2–BRCA1 RING interactions dictate synthesis of mono- or specific polyubiquitin chain linkages
TLDR
BRCA1 has the ability to direct the synthesis of specific polyubiquitin chain linkages, depending on the E2 bound to its RING, according to its ability to bind ubiquitin noncovalently at a site distal to the active site. Expand
Structure of an E3:E2~Ub complex reveals an allosteric mechanism shared among RING/U-box ligases.
TLDR
The model and additional analyses reveal that E3 binding biases dynamic E2~Ub ensembles toward closed conformations with enhanced reactivity for substrate lysines, and identifies a key hydrogen bond between a highly conserved E3 side chain and an E2 backbone carbonyl, observed in all structures of active RING/U-Box E3/E2 pairs, as the linchpin for allosteric activation of E2 ~Ub. Expand
A UbcH5/ubiquitin noncovalent complex is required for processive BRCA1-directed ubiquitination.
TLDR
Self-assembly has profound consequences for the processive formation of polyubiquitin (poly-Ub) chains in ubiquitination reactions directed by the breast and ovarian cancer tumor susceptibility protein BRCA1. Expand
Activation of the bacterial sensor kinase PhoQ by acidic pH.
TLDR
This work proposes a model of activation by antimicrobial peptides via disruption of the cation bridges and/or by acidification of the periplasm through destabilization of the interaction network. Expand
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