Mesolimbic Dopamine Signals the Value of Work
The results indicate that dopamine conveys a single, rapidly evolving decision variable, the available reward for investment of effort, which is employed for both learning and motivational functions.
Temporally resolved catecholamine spikes correspond to single vesicle release from individual chromaffin cells.
- R. Wightman, J. A. Jankowski, O. H. Viveros
- BiologyProceedings of the National Academy of Sciences…
- 1 December 1991
These measurements represent time-resolved observation of quantal secretion of catecholamine and provide direct evidence for the exocytotic hypothesis.
Detection and imaging of zinc secretion from pancreatic beta-cells using a new fluorescent zinc indicator.
- K. Gee, Zhang‐Lin Zhou, W. Qian, R. Kennedy
- Biology, ChemistryJournal of the American Chemical Society
- 12 January 2002
Heterogeneity in secretion among cells was indicated in that some cells in a cluster did not release Zn2+.
Reducing time and increasing sensitivity in sample preparation for adherent mammalian cell metabolomics.
It is demonstrated that a rapid water rinse step prior to quenching of metabolism reduces components that suppress electrospray ionization thereby increasing signal for 26 of 27 targeted metabolites and increasing total number of detected features from 237 to 452 with no detectable change of metabolite content.
Disruption of leptin receptor expression in the pancreas directly affects beta cell growth and function in mice.
Together, these data provide direct genetic evidence for a critical role for leptin signaling in islet biology and suggest that altered leptin action in islets is one factor that contributes to obesity-associated diabetes.
Up-regulation of GLT1 expression increases glutamate uptake and attenuates the Huntington's disease phenotype in the R6/2 mouse
Correlated Oscillations in Glucose Consumption, Oxygen Consumption, and Intracellular Free Ca2+ in Single Islets of Langerhans*
Monitoring of glucose and oxygen levels in the extracellular space of single islets of Langerhans in real-time suggested that during oscillations, bursts of glycolysis begin after Ca2+ has stopped entering the cell, which stimulates increases in respiration.
Dissociable dopamine dynamics for learning and motivation.
A fundamental difference in how dopamine release is regulated to achieve distinct functions is suggested: broadcast burst signals promote learning, whereas local control drives motivation.
Metabolome Response to Glucose in the β-Cell Line INS-1 832/13*
- Matthew A. Lorenz, Mahmoud A. El Azzouny, R. Kennedy, C. Burant
- BiologyJournal of Biological Chemistry
- 20 February 2013
Results include a rapid increase in ATP/ADP, anaplerotic tricarboxylic acid cycle flux, and increases in the malonyl CoA pathway, support prevailing theories of GSIS.