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Specific Substitutions in the Echinocandin Target Fks1p Account for Reduced Susceptibility of Rare Laboratory and Clinical Candida sp. Isolates
TLDR
Results indicate that substitutions in the Fks1p subunit of GS are sufficient to confer reduced susceptibility to echinocandins in S. cerevisiae and the pathogens C. albicans and C. krusei.
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Directed mutagenesis in Candida albicans: one-step gene disruption to isolate ura3 mutants
TLDR
Southern hybridization analyses demonstrated that the U RA3 gene was disrupted on one of the chromosomal homologs in 15 of the 18 transformants analyzed and revealed restriction site dimorphism of EcoRI at the URA3 locus which provides a unique marker to distinguish between chromosomalhomologs.
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An improved protocol for the preparation of yeast cells for transformation by electroporation
TLDR
Pretreatment of yeast cells with lithium acetate and dithiothreitol enhances the frequency of transformation by electroporation and results in 15–300‐fold improvement in transformation frequency with several mutant strains of S. cerevisiae that transformed poorly by conventional procedures.
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A Novel Staphylococcus aureus Vaccine: Iron Surface Determinant B Induces Rapid Antibody Responses in Rhesus Macaques and Specific Increased Survival in a Murine S. aureus Sepsis Model
TLDR
IsdB has excellent prospects for use as a vaccine against S. aureus disease in humans and was highly immunogenic in mice when it was formulated with amorphous aluminum hydroxyphosphate sulfate adjuvant, and the resulting antibody responses were associated with reproducible and significant protection in animal models of infection.
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Isolation of a gene involved in 1,3-beta-glucan synthesis in Aspergillus nidulans and purification of the corresponding protein
TLDR
The product of fksA is important for the activity of highly purified preparations of GS, either as the catalytic subunit itself or as an associated copurifying subunit that mediates susceptibility of enzymatic activity to echinocandin inhibition.
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Geranylgeranyltransferase I of Candida albicans: Null Mutants or Enzyme Inhibitors Produce Unexpected Phenotypes
TLDR
It is hypothesized that Cdc42p and Rho1p of C. albicans can be prenylated by FTase when GGTase I is absent or limiting and that elevation of these two substrates enables them to compete with FTase substrates for prenolation and thus allows sustained growth.
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Transformation of Candida albicans by electroporation.
There is disclosed a procedure for DNA-mediated transformation of Candida albicans by electroporation utilizing lithium acetate and dithiothreitol to weaken the cell wall structure and optimize the…
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One-step gene disruption by cotransformation to isolate double auxotrophs in Candida albicans
TLDR
Southern hybridization analysis revealed that the leu2 mutation reverts and is most likely a point mutation in Candida albicans LEU2; the ade2 ura3 mutant was isolated from the same mutagenesis.
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Purification of geranylgeranyltransferase I from Candida albicans and cloning of the CaRAM2 and CaCDC43 genes encoding its subunits.
TLDR
The authors are investigating the role of geranylgeranylation in the related pathogenic yeast, Candida albicans, which is the most prevalent human fungal pathogen, and finding that hybrid GGTase I heterodimers are nonfunctional.
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Cloning and characterization of the 2,3-oxidosqualene cyclase-coding gene of Candida albicans.
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