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The Genome Sequence of the Metal-Mobilizing, Extremely Thermoacidophilic Archaeon Metallosphaera sedula Provides Insights into Bioleaching-Associated Metabolism
Comparisons with the mesophilic biomining bacterium Acidithiobacillus ferrooxidans indicate that the M. sedula genome encodes at least one putative rusticyanin, involved in iron oxidation, and a putative tetrathionate hydrolase, implicated in sulfur oxidation, which is a desirable trait of biomining microorganisms.
Microbial biochemistry, physiology, and biotechnology of hyperthermophilic Thermotoga species.
Structural genomics efforts aimed at the model hyperthermophile Thermotoga maritima proteome have yielded hundreds of high-resolution datasets and predicted functions for uncharacterized proteins, particularly for carbohydrate utilization and iron-sulfur cluster synthesis and repair.
Hydrogenomics of the Extremely Thermophilic Bacterium Caldicellulosiruptor saccharolyticus
W Whole-genome transcriptome analysis revealed that there is significant upregulation of the glycolytic pathway and an ABC-type sugar transporter during growth on glucose and xylose, indicating that C. saccharolyticus coferments these sugars unimpeded by glucose-based catabolite repression.
Phylogenetic, Microbiological, and Glycoside Hydrolase Diversities within the Extremely Thermophilic, Plant Biomass-Degrading Genus Caldicellulosiruptor
Overall, the genus Caldicellulosiruptor appears to contain more genomic and physiological diversity than previously reported, and this argues for continued efforts to isolate new members from high-temperature terrestrial biotopes.
An Expression-Driven Approach to the Prediction of Carbohydrate Transport and Utilization Regulons in theHyperthermophilic Bacterium Thermotoga maritima
The results indicate that T. maritima relies extensively on ABC transporters for carbohydrate uptake, many of which are likely controlled by local regulators responsive to either the transport substrate or a key metabolic degradation product.
Global analysis of carbohydrate utilization by Lactobacillus acidophilus using cDNA microarrays.
The transport and catabolic machinery involved in carbohydrate utilization by Lactobacillus acidophilus was characterized genetically by using whole-genome cDNA microarrays, and genes central to glycolysis were among the most highly expressed in the genome.
Purification and characterization of extremely thermostable beta-mannanase, beta-mannosidase, and alpha-galactosidase from the hyperthermophilic eubacterium Thermotoga neapolitana 5068
These enzymes represent the most thermostable and thermoactive versions of these types yet reported and probably act synergistically to hydrolyze extracellular galactomannans to monosaccharides by T. neapolitana for nutritional purposes.
Comparison of a β-Glucosidase and a β-Mannosidase from the Hyperthermophilic Archaeon Pyrococcus furiosus
Two distinct exo-acting, β-specific glycosyl hydrolases were purified to homogeneity from crude cell extracts of the hyperthermophilic archaeon Pyrococcus furiosus: a β-glucosidase, corresponding to
Sequence, expression in Escherichia coli, and analysis of the gene encoding a novel intracellular protease (PfpI) from the hyperthermophilic archaeon Pyrococcus furiosus
A previously identified intracellular proteolytic activity in the hyperthermophilic archaeon Pyrococcus furiosus was found to be a homomultimer consisting of 18.8-kDa subunits; an immunological relationship of this protease to the eukaryotic proteasome has been seen previously.