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Engineering the third wave of biocatalysis
TLDR
Applications of protein-engineered biocatalysts ranging from commodity chemicals to advanced pharmaceutical intermediates that use enzyme catalysis as a key step are discussed.
Hydrolases in Organic Synthesis: Regio- and Stereoselective Biotransformations
TLDR
Using Enantioselective Reactions to Design and Optimize Biocatalyst Performance and Survey of EnantiOSElective Protease-and Amidase-Catalyzed Reactions.
How the Same Core Catalytic Machinery Catalyzes 17 Different Reactions: the Serine-Histidine-Aspartate Catalytic Triad of α/β-Hydrolase Fold Enzymes.
TLDR
Detailed analysis of the subset of α/β-hydrolase fold enzymes that contain the serine-histidine-aspartate catalytic triad shows that binding differences and non-catalytic residues create new mechanisms and are essential for understanding and designing efficient enzymes.
Biocatalysis in ionic liquids - advantages beyond green technology.
Improved preparation and use of room-temperature ionic liquids in lipase-catalyzed enantio- and regioselective acylations.
TLDR
Ionic liquids permit reactions in a more polar solvent than previously possible, and lipase-catalyzed reactions that previously did not occur in untreated ionic liquids now occur at rates comparable to those in nonpolar organic solvents such as toluene.
Hydrolase-catalyzed biotransformations in deep eutectic solvents.
Hydrolases show good catalytic activity in deep eutectic solvents, despite the presence of urea, which can denature enzymes, or alcohols, which can interfere with hydrolase-catalyzed reactions.
Toward advanced ionic liquids. Polar, enzyme-friendly solvents for biocatalysis
TLDR
This review covers selected examples of enzyme catalyzed reaction in ionic liquids that demonstrate their advantages and unique properties, and point out opportunities for new applications.
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