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In vitro biosynthesis of poly(3-hydroxybutyric acid) by using purified poly(hydroxyalkanoic acid) synthase of Chromatium vinosum
- R. Jossek, R. Reichelt, A. Steinbüchel
- Chemistry, Medicine
- Applied Microbiology and Biotechnology
- 1 March 1998
Abstract Purified recombinant poly(hydroxyalkanoic acid) (PHA) synthase from Chromatium vinosum (PhaECCv) was used to examine in vitro the specific synthase activity, turnover of… Expand
Characterization of a new feedback-resistant 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase AroF of Escherichia coli.
It is shown that an Asn-8 to Lys-8 substitution in AroF leads to a tyrosine-insensitive DAHP synthase, which strongly suggest that the N-terminus of A roF is involved in the molecular interactions occurring in the feedback-inhibition mechanism. Expand
Isolation and characterization of maltokinase (ATP:maltose 1-phosphotransferase) from Actinoplanes missouriensis
Tryptic peptides of the enzyme were sequenced, and the sequences of these peptides will allow construction of degenerate primers to identify the gene coding for this unique kinase. Expand
In vitro synthesis of poly(3-hydroxybutyric acid) by using an enzymatic coenzyme A recycling system.
A handy system is available to produce in vitro PHA on a semipreparative scale by using purified recombinant poly(hydroxyalkanoic acid), PHA, synthase from Chromatium vinosum to examine in vitro poly(3-hydroxybutyric acid) (P(3HB)) formation. Expand
Biosynthesis of polyesters in bacteria and recombinant organisms
Abstract Many bacteria are able to synthesize polyesters of hydroxyalkanoic acids (PHA), which occur as insoluble cytoplasmic inclusions in the cell and can contribute significantly to the cellular… Expand
Metabolic engineering to produce fine chemicals in Escherichia coli.