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Prosthetic groups of the NADH-dependent nitrite reductase from Escherichia coli K12.
A substantially improved purification of Escherichia coli NADH-dependent nitrite reductase was obtained by purifying it in presence of 1 mM-NO2- and 10 microM-FAD. The enzyme was obtained in 20%Expand
Ribosome display and related technologies : methods and protocols
Part I: Review 1. Ribosome Display: A Perspective Andreas Pluckthun Part II: Translation Extract Preparation 2. Preparation and Testing of E.coli S30 In Vitro Transcription Translation Extracts JamesExpand
The steady-state kinetics of the NADH-dependent nitrite reductase from Escherichia coli K 12. Nitrite and hydroxylamine reduction.
The reduction of both NO2- and hydroxylamine by the NADH-dependent nitrite reductase of Escherichia coli K 12 (EC 1.6.6.4) appears to follow Michaelis-Menten kinetics over a wide range of NADHExpand
The mucoprotein of the fat/plasma interface of cow's milk. I. Chemical and physical characterization.
Abstract A mucoprotein isolated from the fat/plasma interface of cow's milk has been characterized. As analyzed by free-boundary electrophoresis, the mucoprotein was essentially homogeneous withExpand
Highly efficient ribosome display selection by use of purified components for in vitro translation.
Ribosome display is a powerful in vitro technology for the selection and directed evolution of proteins. The ribosome display process exploits cell-free translation to achieve coupling of phenotypeExpand
Inactivation of the 2-ketoglutarate and pyruvate dehydrogenase complexes of beef heart by branched chain keto acids.
Incubation of 2-ketoglutarate dehydrogenase complex with 2-ketoisovalerate, 2-keto-4-methylvalerate, or 2-keto-3-methylvalerate leads to the appearance of a lag phase and of a progressive loss ofExpand
Phage-enzymes: expression and affinity chromatography of functional alkaline phosphatase on the surface of bacteriophage.
We have demonstrated that an active enzyme can be expressed on the surface of a bacteriophage. The gene encoding alkaline phosphatase from Escherichia coli was cloned upstream of gene 3, whichExpand
The mucoprotein of the fat/plasma interface of cow's milk. II. Immunochemical characterization.
Abstract The sialic acid-containing mucoprotein from the fat/plasma interface of cow's milk functioned as a strong antigen. The mucoprotein preparation induced formation of antibodies in rabbits toExpand
Affinity maturation of phage display antibody populations using ribosome display.
A comparison has been performed, using phage display or ribosome display, of stringent selections on antibody populations derived from three rounds of phage display selection. Stringent selectionsExpand
Electron-spin-resonance studies of the NADH-dependent nitrite reductase from Escherichia coli K12.
The NADH-dependent nitrite reductase of Escherichia coli, which contains sirohaem, flavin, non-haem iron and labile sulphide, was examined by low-temperature e.s.r. spectroscopy. The enzyme, storedExpand
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