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A Functional Genomics Approach to Tanshinone Biosynthesis Provides Stereochemical Insights
TLDR
The initial results from a functional genomics-based investigation of tanshinone biosynthesis, specifically the functional identification of the relevant diterpene synthases from S. miltiorrhiza Bunge, show the cyclohexa-1,4-diene arrangement of the distal ring poises the resulting miltiradiene for the ensuing aromatization and hydroxylation to ferruginol suggested forTanshinones. Expand
CYP76AH1 catalyzes turnover of miltiradiene in tanshinones biosynthesis and enables heterologous production of ferruginol in yeasts
TLDR
The results and the approaches that were described here provide a solid foundation to further elucidate the biosynthesis of tanshinones and related diterpenoids and should facilitate the construction of microbial cell factories for the production of phytoterpenoids. Expand
The Maize An2 Gene is Induced by Fusarium Attack and Encodes an ent-Copalyl Diphosphate Synthase
TLDR
Using the technique of differential display, a maize transcript was identified whose silk tissue expression is induced in the presence of the ear rot pathogen Fusarium graminearum, and it was demonstrated that both AN1 and AN2 are ent-copalyl diphosphate (ent-CPP) synthases (ent -CPS). Expand
Terpenoid synthase structures: a so far incomplete view of complex catalysis.
TLDR
The complexity of terpenoid natural products has drawn significant interest, and while this has certainly led to some insights into the enzymatic structure-function relationships underlying the elongation and simpler cyclization reactions, the understanding of the more complex cyclization and/or rearrangement reactions remains limited. Expand
Rice Contains Two Disparate ent-Copalyl Diphosphate Synthases with Distinct Metabolic Functions1
TLDR
A functional genomics approach has been utilized to identify two disparate ent-copalyl diphosphate synthases from rice (OsCPS1ent and OsCPS2ent) and evidence is presented here strongly indicating that the other is involved in related secondary metabolism producing defensive phytochemicals. Expand
Full-length transcriptome sequences and splice variants obtained by a combination of sequencing platforms applied to different root tissues of Salvia miltiorrhiza and tanshinone biosynthesis.
TLDR
It is demonstrated that the tanshinone pigments are produced and accumulate in the root periderm, and a combination of NGS and single-molecule real-time (SMRT) sequencing to various root tissues is applied to provide a more complete view of the S. miltiorrhiza transcriptome. Expand
Biosynthesis, elicitation and roles of monocot terpenoid phytoalexins.
TLDR
Recent advances in the discovery, biosynthesis, elicitation and functional characterization of monocot terpenoid phytoalexins are discussed, including conclusive evidence now exists for multiple plant receptors of fungal-derived chitin elicitors, phosphorylation of membrane-associated signaling complexes, activation of mitogen-activated protein kinase, involvement of phytohormone signals, and the existence of transcription factors. Expand
Investigation of terpene diversification across multiple sequenced plant genomes
TLDR
Focusing on the terpenes, the largest class of plant natural products, the basis of terpene diversity is investigated through analysis of multiple sequenced plant genomes and evidence is found for different mechanisms of pathway assembly in eudicots and monocots. Expand
A modular approach for facile biosynthesis of labdane-related diterpenes.
TLDR
A modular approach for facile biosynthesis of labdane-related diterpenes wherein base pGGxC vectors capable of introducing bacterial production of any one of the three common stereoisomers of CPP can be co-introduced with diterpene synthases that convert these CPP intermediates to specific diterPene hydrocarbon skeletal structures. Expand
Monoterpene biosynthesis pathway construction in Escherichia coli.
TLDR
Feeding studies with (-)-limonene, to overcome the flux deficiency, demonstrated the functional capability of limonene-6-hydroxylase and carveol dehydrogenase to produce the end-product carvone; however, uptake and trafficking restrictions greatly compromised the efficiency of these conversions. Expand
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